Temperature-Tolerated Mainstream Nitrogen Removal by Anammox and Nitrite/Nitrate-Dependent Anaerobic Methane Oxidation in a Membrane Biofilm Reactor

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Tao Liu
  • Zhuan Khai Lim
  • Hui Chen
  • Shihu Hu
  • Jianhua Guo

Detail(s)

Original languageEnglish
Pages (from-to)3012-3021
Journal / PublicationEnvironmental Science and Technology
Volume54
Issue number5
Online published8 Feb 2020
Publication statusPublished - 3 Mar 2020
Externally publishedYes

Abstract

The mainstream anaerobic ammonium oxidation (anammox) process provides strong support to the on-going paradigm shift from energy-negative to energy-neutral in wastewater treatment plants. However, the low temperature (e.g., below 15 °C) represents one of the major challenges for mainstream anammox in practice. In this study, a stable nitrogen removal rate (0.13 kg m-3 day-1), together with a high-level effluent quality (<5.0 mg N L-1), was achieved in a lab-scale upflow membrane biofilm reactor (MBfR) by coupling anammox with nitrite/nitrate-dependent anaerobic methane oxidation (n-DAMO) microorganisms, at a temperature as low as 10 °C. With the temperature being progressively decreased from 25 to 10 °C, the total nitrogen removal efficiency was maintained in the range of 90-94% at a constant hydraulic retention time of 9 h. The impact of temperature on the biofilm system coupling anammox and n-DAMO reactions increased at a lower temperature range with higher Arrhenius coefficients. Additionally, 16S rRNA gene sequencing results showed that anammox bacteria, n-DAMO bacteria, and n-DAMO archaea jointly dominated the biofilm, and their respective abundances remained relatively stable when the temperature was decreased. The major reason for this temperature-tolerated performance is the overcapacity developed, which is indicated by biofilm thickness measurements and mathematical modeling. The stable performance obtained in this study shows promise for the n-DAMO application in domestic wastewater.  © 2020 American Chemical Society.

Citation Format(s)

Temperature-Tolerated Mainstream Nitrogen Removal by Anammox and Nitrite/Nitrate-Dependent Anaerobic Methane Oxidation in a Membrane Biofilm Reactor. / Liu, Tao; Khai Lim, Zhuan; Chen, Hui et al.
In: Environmental Science and Technology, Vol. 54, No. 5, 03.03.2020, p. 3012-3021.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review