Targeting Nrf2-mediated heme oxygenase-1 enhances non-thermal plasma-induced cell death in non-small-cell lung cancer A549 cells

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

11 Scopus Citations
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Author(s)

  • Jie Ma
  • Cheng Cheng
  • Guohua Ni
  • Jie Shen
  • Wei Han

Related Research Unit(s)

Detail(s)

Original languageEnglish
Pages (from-to)54-65
Journal / PublicationArchives of Biochemistry and Biophysics
Volume658
Online published21 Sep 2018
Publication statusPublished - 15 Nov 2018

Abstract

Non-thermal plasma (NTP) treatment has been proposed as a potential approach for cancer therapy for killing cancer cells via generation of reactive oxygen species (ROS). As an antioxidant protein, Heme oxygenase-1 (HO-1) has been known to protect cells against oxidative stress. In this paper, we investigated the role of HO-1 activation in NTP-induced apoptosis in A549 cells. Distinctly increased ROS production and apoptosis were observed after NTP exposure. NTP exposure induced HO-1 expression in a dose- and time-dependent manner via activating the translocation of Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) from cytoplasm to nucleus. Furthermore, inhibiting HO-1 activation with its specific inhibitor, ZnPP, increased “killing” effect of NTP. Knocking down HO-1 or Nrf2 with the special siRNA also led to elevated ROS level and enhanced NTP-induced cell death. In addition, the c-JUN N-terminal kinase (JNK) signaling pathway was shown to be involved in NTP-induced HO-1 expression. Interestingly, a higher resistance to NTP exposure of A549 cell compared to H1299 and H322 cells was found to be linked to its higher basal level of HO-1 expression. These findings revealed that HO-1 could be considered as a potential target to improve the effect of NTP in cancer therapy.

Research Area(s)

  • Apoptotic death, c-JUN N-terminal kinase, Heme oxygenase-1, Non-thermal plasma, Reactive oxygen species

Citation Format(s)

Targeting Nrf2-mediated heme oxygenase-1 enhances non-thermal plasma-induced cell death in non-small-cell lung cancer A549 cells. / Ma, Jie; Yu, K. N.; Cheng, Cheng; Ni, Guohua; Shen, Jie; Han, Wei.

In: Archives of Biochemistry and Biophysics, Vol. 658, 15.11.2018, p. 54-65.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review