Targeted detection of g-quadruplexes in cellular RNAs

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

45 Scopus Citations
View graph of relations

Author(s)

Detail(s)

Original languageEnglish
Pages (from-to)6751-6754
Journal / PublicationAngewandte Chemie - International Edition
Volume54
Issue number23
Online published23 Apr 2015
Publication statusPublished - 1 Jun 2015
Externally publishedYes

Abstract

The G-quadruplex (G4) is a non-canonical nucleic acid structure which regulates important cellular processes. RNA G4s have recently been shown to exist in human cells and be biologically significant. Described herein is a new approach to detect and map RNA G4s in cellular transcripts. This method exploits the specific control of RNA G4-cation and RNA G4-ligand interactions during reverse transcription, by using a selective reverse transcriptase to monitor RNA G4-mediated reverse transcriptase stalling (RTS) events. Importantly, a ligation-amplification strategy is coupled with RTS, and enables detection and mapping of G4s in important, low-abundance cellular RNAs. Strong evidence is provided for G4 formation in full-length cellular human telomerase RNA, offering important insights into its cellular function.

Research Area(s)

  • cations, G-quadruplexes, ligand effects, reverse transcription, RNA structures, telomerase RNA

Citation Format(s)

Targeted detection of g-quadruplexes in cellular RNAs. / Kwok, Chun Kit; Balasubramanian, Shankar.

In: Angewandte Chemie - International Edition, Vol. 54, No. 23, 01.06.2015, p. 6751-6754.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review