TAG pathway engineering via GPAT2 concurrently potentiates abiotic stress tolerance and oleaginicity in Phaeodactylum tricornutum

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Xiang Wang
  • Si-Fen Liu
  • Ruo-Yu Li
  • Wei-Dong Yang
  • Jie-Sheng Liu
  • Srinivasan Balamurugan
  • Hong-Ye Li

Related Research Unit(s)

Detail(s)

Original languageEnglish
Article number160
Journal / PublicationBiotechnology for Biofuels
Volume13
Online published14 Sep 2020
Publication statusPublished - 2020

Link(s)

Abstract

Background: Despite the great potential of marine diatoms in biofuel sector, commercially viable biofuel production from native diatom strain is impractical. Targeted engineering of TAG pathway represents a promising approach; however, recruitment of potential candidate has been regarded as critical. Here, we identified a glycerol-3-phosphate acyltransferase 2 (GPAT2) isoform and overexpressed in Phaeodactylum tricornutum
Results: GPAT2 overexpression did not impair growth and photosynthesis. GPAT2 overexpression reduced carbohydrates and protein content, however, lipid content were significantly increased. Specifically, TAG content was notably increased by 2.9-fold than phospho-and glyco-lipids. GPAT2 overexpression elicited the push-And-pull strategy by increasing the abundance of substrates for the subsequent metabolic enzymes, thereby increased the expression of LPAAT and DGAT. Besides, GPAT2-mediated lipid overproduction coordinated the expression of NADPH biosynthetic genes. GPAT2 altered the fatty acid profile in TAGs with C16:0 as the predominant fatty acid moieties. We further investigated the impact of GPAT2 on conferring abiotic stress, which exhibited enhanced tolerance to hyposaline (70%) and chilling (10 ºC) conditions via altered fatty acid saturation level. 
Conclusions: Collectively, our results exemplified the critical role of GPAT2 in hyperaccumulating TAGs with altered fatty acid profile, which in turn uphold resistance to abiotic stress conditions.

Research Area(s)

  • Abiotic stress tolerance, Diatom, Glycerol-3-phosphate acyltransferase, Lipid hyperaccumulation, Lipid remodeling

Citation Format(s)

TAG pathway engineering via GPAT2 concurrently potentiates abiotic stress tolerance and oleaginicity in Phaeodactylum tricornutum. / Wang, Xiang; Liu, Si-Fen; Li, Ruo-Yu; Yang, Wei-Dong; Liu, Jie-Sheng; Lin, Carol Sze Ki; Balamurugan, Srinivasan; Li, Hong-Ye.

In: Biotechnology for Biofuels, Vol. 13, 160, 2020.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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