TY - JOUR
T1 - Taenia solium porcine cysticercosis in Madagascar
T2 - Comparison of immuno-diagnostic techniques and estimation of the prevalence in pork carcasses traded in Antananarivo city
AU - Porphyre, V.
AU - Betson, M.
AU - Rabezanahary, H.
AU - Mboussou, Y.
AU - Zafindraibe, N. J.
AU - Rasamoelina-Andriamanivo, H.
AU - Costard, S.
AU - Pfeiffer, D.U
AU - Michault, A.
PY - 2016/3/30
Y1 - 2016/3/30
N2 - Taenia solium cysticercosis was reported in official veterinary and medical statistics to be highly prevalent in pigs and humans in Madagascar, but few estimates are available for pigs. This study aimed to estimate the seroprevalence of porcine cysticercosis among pigs slaughtered in Antananarivo abattoirs. Firstly, the diagnostic performance of two antigen-ELISA techniques (B158B60 Ag-ELISA and HP10 Ag-ELISA) and an immunoblotting method were compared with meat inspection procedures on a sample of pigs suspected to be infected with (group 1; n = 250) or free of (group 2; n = 250) T. solium based on direct veterinary inspection in Madagascar. Sensitivity and specificity of the antigen ELISAs were then estimated using a Bayesian approach for detection of porcine cysticercosis in the absence of a gold standard. Then, a third set of pig sera (group 3, n = 250) was randomly collected in Antananarivo slaughterhouses and tested to estimate the overall prevalence of T. solium contamination in pork meat traded in Antananarivo.The antigen ELISAs showed a high sensitivity (
AB - Taenia solium cysticercosis was reported in official veterinary and medical statistics to be highly prevalent in pigs and humans in Madagascar, but few estimates are available for pigs. This study aimed to estimate the seroprevalence of porcine cysticercosis among pigs slaughtered in Antananarivo abattoirs. Firstly, the diagnostic performance of two antigen-ELISA techniques (B158B60 Ag-ELISA and HP10 Ag-ELISA) and an immunoblotting method were compared with meat inspection procedures on a sample of pigs suspected to be infected with (group 1; n = 250) or free of (group 2; n = 250) T. solium based on direct veterinary inspection in Madagascar. Sensitivity and specificity of the antigen ELISAs were then estimated using a Bayesian approach for detection of porcine cysticercosis in the absence of a gold standard. Then, a third set of pig sera (group 3, n = 250) was randomly collected in Antananarivo slaughterhouses and tested to estimate the overall prevalence of T. solium contamination in pork meat traded in Antananarivo.The antigen ELISAs showed a high sensitivity (
KW - Cysticercosis
KW - ELISA
KW - Enzyme-linked immunoelectrotransfer blot
KW - Immunodiagnostic
KW - Madagascar
KW - Pigs
KW - Taenia solium
UR - http://www.scopus.com/inward/record.url?scp=84960080617&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84960080617&origin=recordpage
U2 - 10.1016/j.vetpar.2015.08.027
DO - 10.1016/j.vetpar.2015.08.027
M3 - RGC 21 - Publication in refereed journal
C2 - 26342625
SN - 0304-4017
VL - 219
SP - 77
EP - 83
JO - Veterinary Parasitology
JF - Veterinary Parasitology
ER -