Taenia solium porcine cysticercosis in Madagascar: Comparison of immuno-diagnostic techniques and estimation of the prevalence in pork carcasses traded in Antananarivo city

V. Porphyre; M. Betson; H. Rabezanahary; Y. Mboussou; N. J. Zafindraibe; H. Rasamoelina-Andriamanivo; S. Costard; D.U Pfeiffer; A. Michault

doi:10.1016/j.vetpar.2015.08.027

Author(s)

V. Porphyre
M. Betson
H. Rabezanahary
Y. Mboussou
N. J. Zafindraibe
H. Rasamoelina-Andriamanivo
S. Costard
A. Michault

Detail(s)

Original language	English
Pages (from-to)	77-83
Journal / Publication	Veterinary Parasitology
Volume	219
Publication status	Published - 30 Mar 2016
Externally published	Yes

Link(s)

DOI	DOI https://doi.org/10.1016/j.vetpar.2015.08.027 Final Published version
	Check@CityULib
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-84960080617&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(7919bb6d-78a4-4e89-9519-6741b164f84b).html

Abstract

Taenia solium cysticercosis was reported in official veterinary and medical statistics to be highly prevalent in pigs and humans in Madagascar, but few estimates are available for pigs. This study aimed to estimate the seroprevalence of porcine cysticercosis among pigs slaughtered in Antananarivo abattoirs. Firstly, the diagnostic performance of two antigen-ELISA techniques (B158B60 Ag-ELISA and HP10 Ag-ELISA) and an immunoblotting method were compared with meat inspection procedures on a sample of pigs suspected to be infected with (group 1; n = 250) or free of (group 2; n = 250) T. solium based on direct veterinary inspection in Madagascar. Sensitivity and specificity of the antigen ELISAs were then estimated using a Bayesian approach for detection of porcine cysticercosis in the absence of a gold standard. Then, a third set of pig sera (group 3, n = 250) was randomly collected in Antananarivo slaughterhouses and tested to estimate the overall prevalence of T. solium contamination in pork meat traded in Antananarivo.The antigen ELISAs showed a high sensitivity (

Research Area(s)

Cysticercosis, ELISA, Enzyme-linked immunoelectrotransfer blot, Immunodiagnostic, Madagascar, Pigs, Taenia solium

SDG 17 - Partnerships for the Goals

Citation Format(s)

[ RIS ] [ BibTeX ]

Taenia solium porcine cysticercosis in Madagascar: Comparison of immuno-diagnostic techniques and estimation of the prevalence in pork carcasses traded in Antananarivo city. / Porphyre, V.; Betson, M.; Rabezanahary, H. et al.
In: Veterinary Parasitology, Vol. 219, 30.03.2016, p. 77-83.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

Porphyre, V, Betson, M, Rabezanahary, H, Mboussou, Y, Zafindraibe, NJ, Rasamoelina-Andriamanivo, H, Costard, S, Pfeiffer, DU & Michault, A 2016, 'Taenia solium porcine cysticercosis in Madagascar: Comparison of immuno-diagnostic techniques and estimation of the prevalence in pork carcasses traded in Antananarivo city', Veterinary Parasitology, vol. 219, pp. 77-83. https://doi.org/10.1016/j.vetpar.2015.08.027

Porphyre, V., Betson, M., Rabezanahary, H., Mboussou, Y., Zafindraibe, N. J., Rasamoelina-Andriamanivo, H., Costard, S., Pfeiffer, D. U., & Michault, A. (2016). Taenia solium porcine cysticercosis in Madagascar: Comparison of immuno-diagnostic techniques and estimation of the prevalence in pork carcasses traded in Antananarivo city. Veterinary Parasitology, 219, 77-83. https://doi.org/10.1016/j.vetpar.2015.08.027

Porphyre V, Betson M, Rabezanahary H, Mboussou Y, Zafindraibe NJ, Rasamoelina-Andriamanivo H et al. Taenia solium porcine cysticercosis in Madagascar: Comparison of immuno-diagnostic techniques and estimation of the prevalence in pork carcasses traded in Antananarivo city. Veterinary Parasitology. 2016 Mar 30;219:77-83. doi: 10.1016/j.vetpar.2015.08.027

Porphyre, V. ; Betson, M. ; Rabezanahary, H. et al. / Taenia solium porcine cysticercosis in Madagascar : Comparison of immuno-diagnostic techniques and estimation of the prevalence in pork carcasses traded in Antananarivo city. In: Veterinary Parasitology. 2016 ; Vol. 219. pp. 77-83.

Taenia solium porcine cysticercosis in Madagascar : Comparison of immuno-diagnostic techniques and estimation of the prevalence in pork carcasses traded in Antananarivo city