Synthetic nanostructures inducing differentiation of human mesenchymal stem cells into neuronal lineage
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Author(s)
Detail(s)
Original language | English |
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Pages (from-to) | 1820-1829 |
Journal / Publication | Experimental Cell Research |
Volume | 313 |
Issue number | 9 |
Publication status | Published - 15 May 2007 |
Externally published | Yes |
Link(s)
Abstract
Human mesenchymal stem cells (hMSCs) have been shown to trans-differentiate into neuronal-like cells by culture in neuronal induction media, although the mechanism is not well understood. Topography can also influence cellular responses including enhanced differentiation of progenitor cells. As extracellular matrix (ECM) in vivo comprises topography in the nanoscale, we hypothesize that nanotopography could influence stem cell differentiation into specific non-default pathways, such as transdifferentiation of hMSCs. Differentiation and proliferation of hMSCs were studied on nanogratings of 350 nm width. Cytoskeleton and nuclei of hMSCs were aligned and elongated along the nanogratings. Gene profiling and immunostaining showed significant up-regulation of neuronal markers such as microtubule-associated protein 2 (MAP2) compared to unpatterned and micropatterned controls. The combination of nanotopography and biochemical cues such as retinoic acid further enhanced the up-regulation of neuronal marker expressions, but nanotopography showed a stronger effect compared to retinoic acid alone on unpatterned surface. This study demonstrated the significance of nanotopography in directing differentiation of adult stem cells. © 2007 Elsevier Inc. All rights reserved.
Research Area(s)
- Cytoskeleton rearrangement, Human mesenchymal stem cells, Nanoimprinting, Nanotopography, Neuronal differentiation
Citation Format(s)
Synthetic nanostructures inducing differentiation of human mesenchymal stem cells into neuronal lineage. / Yim, Evelyn K.F.; Pang, Stella W.; Leong, Kam W.
In: Experimental Cell Research, Vol. 313, No. 9, 15.05.2007, p. 1820-1829.
In: Experimental Cell Research, Vol. 313, No. 9, 15.05.2007, p. 1820-1829.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review