Synthetic Hexadecapeptide Prevents Postharvest Pectobacterium carotovorum (subsp. brasiliensis BC1) Infection via Destabilizing Cell Envelope and Suppressing Biosynthesis of Arginine and Peptidoglycan

Ping Zeng; Lanhua Yi; Melody Wong; Sheng Chen; Kin-Fai Chan; Kwok-Yin Wong

doi:10.1021/acsfoodscitech.1c00037

Synthetic Hexadecapeptide Prevents Postharvest Pectobacterium carotovorum (subsp. brasiliensis BC1) Infection via Destabilizing Cell Envelope and Suppressing Biosynthesis of Arginine and Peptidoglycan

Ping Zeng
, Lanhua Yi
, Melody Wong
, Sheng Chen^*
, Kin-Fai Chan^*
, Kwok-Yin Wong^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

4 Citations (Scopus)

Abstract

Pectobacterium carotovorum is a Gram-negative plant-specific pathogen that causes severe rot of postharvest vegetables. Given the safety consideration of pesticides, we developed a peptide approach to address this issue. This hexadecapeptide (zp16) inhibited the growth of planktonic bacterial cells at micromolar concentration and reduced the live bacterial count in a cabbage leaf. Mechanistic studies revealed that zp16 rapidly influenced membrane potential and permeability and triggered significant reactive oxygen species accumulation. Furthermore, proteomics analysis suggested that the expression of some critical proteins related to arginine and peptidoglycan biosynthesis was statistically significantly down-regulated after transitory zp16 treatment. Collectively, zp16 kills the bacteria not only by physically damaging the cellular envelope but also interfering with multiple metabolic processes.

Original language	English
Pages (from-to)	614-624
Journal	ACS Food Science & Technology
Volume	1
Issue number	4
Online published	13 Apr 2021
DOIs	https://doi.org/10.1021/acsfoodscitech.1c00037
Publication status	Published - 21 May 2021

Research Keywords

antimicrobial peptide
Pectobacterium carotovorum
soft rot
membrane potential
reactive oxygen species
proteomic analysis

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10.1021/acsfoodscitech.1c00037

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CRF: Development of Novel Inhibitors Targeting the Resistance Mechanisms of Clinical Superbugs
CHEN, S. (Principal Investigator / Project Coordinator), CHAN, K. F. (Co-Principal Investigator), Hao, Q. (Co-Principal Investigator), LI, X. (Co-Principal Investigator) & MA, E.D.-L. (Co-Principal Investigator)
30/06/17 → 29/06/21
Project: Research

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@article{bec6f4dccfd3420bbd5e1ff78e8f6483,

title = "Synthetic Hexadecapeptide Prevents Postharvest Pectobacterium carotovorum (subsp. brasiliensis BC1) Infection via Destabilizing Cell Envelope and Suppressing Biosynthesis of Arginine and Peptidoglycan",

abstract = "Pectobacterium carotovorum is a Gram-negative plant-specific pathogen that causes severe rot of postharvest vegetables. Given the safety consideration of pesticides, we developed a peptide approach to address this issue. This hexadecapeptide (zp16) inhibited the growth of planktonic bacterial cells at micromolar concentration and reduced the live bacterial count in a cabbage leaf. Mechanistic studies revealed that zp16 rapidly influenced membrane potential and permeability and triggered significant reactive oxygen species accumulation. Furthermore, proteomics analysis suggested that the expression of some critical proteins related to arginine and peptidoglycan biosynthesis was statistically significantly down-regulated after transitory zp16 treatment. Collectively, zp16 kills the bacteria not only by physically damaging the cellular envelope but also interfering with multiple metabolic processes.",

keywords = "antimicrobial peptide, Pectobacterium carotovorum, soft rot, membrane potential, reactive oxygen species, proteomic analysis",

author = "Ping Zeng and Lanhua Yi and Melody Wong and Sheng Chen and Kin-Fai Chan and Kwok-Yin Wong",

year = "2021",

month = may,

day = "21",

doi = "10.1021/acsfoodscitech.1c00037",

language = "English",

volume = "1",

pages = "614--624",

number = "4",

}

Zeng, P, Yi, L, Wong, M, Chen, S, Chan, K-F & Wong, K-Y 2021, 'Synthetic Hexadecapeptide Prevents Postharvest Pectobacterium carotovorum (subsp. brasiliensis BC1) Infection via Destabilizing Cell Envelope and Suppressing Biosynthesis of Arginine and Peptidoglycan', ACS Food Science & Technology, vol. 1, no. 4, pp. 614-624. https://doi.org/10.1021/acsfoodscitech.1c00037

Synthetic Hexadecapeptide Prevents Postharvest Pectobacterium carotovorum (subsp. brasiliensis BC1) Infection via Destabilizing Cell Envelope and Suppressing Biosynthesis of Arginine and Peptidoglycan. / Zeng, Ping; Yi, Lanhua; Wong, Melody et al.
In: ACS Food Science & Technology, Vol. 1, No. 4, 21.05.2021, p. 614-624.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

TY - JOUR

T1 - Synthetic Hexadecapeptide Prevents Postharvest Pectobacterium carotovorum (subsp. brasiliensis BC1) Infection via Destabilizing Cell Envelope and Suppressing Biosynthesis of Arginine and Peptidoglycan

AU - Zeng, Ping

AU - Yi, Lanhua

AU - Wong, Melody

AU - Chen, Sheng

AU - Chan, Kin-Fai

AU - Wong, Kwok-Yin

PY - 2021/5/21

Y1 - 2021/5/21

N2 - Pectobacterium carotovorum is a Gram-negative plant-specific pathogen that causes severe rot of postharvest vegetables. Given the safety consideration of pesticides, we developed a peptide approach to address this issue. This hexadecapeptide (zp16) inhibited the growth of planktonic bacterial cells at micromolar concentration and reduced the live bacterial count in a cabbage leaf. Mechanistic studies revealed that zp16 rapidly influenced membrane potential and permeability and triggered significant reactive oxygen species accumulation. Furthermore, proteomics analysis suggested that the expression of some critical proteins related to arginine and peptidoglycan biosynthesis was statistically significantly down-regulated after transitory zp16 treatment. Collectively, zp16 kills the bacteria not only by physically damaging the cellular envelope but also interfering with multiple metabolic processes.

AB - Pectobacterium carotovorum is a Gram-negative plant-specific pathogen that causes severe rot of postharvest vegetables. Given the safety consideration of pesticides, we developed a peptide approach to address this issue. This hexadecapeptide (zp16) inhibited the growth of planktonic bacterial cells at micromolar concentration and reduced the live bacterial count in a cabbage leaf. Mechanistic studies revealed that zp16 rapidly influenced membrane potential and permeability and triggered significant reactive oxygen species accumulation. Furthermore, proteomics analysis suggested that the expression of some critical proteins related to arginine and peptidoglycan biosynthesis was statistically significantly down-regulated after transitory zp16 treatment. Collectively, zp16 kills the bacteria not only by physically damaging the cellular envelope but also interfering with multiple metabolic processes.

KW - antimicrobial peptide

KW - Pectobacterium carotovorum

KW - soft rot

KW - membrane potential

KW - reactive oxygen species

KW - proteomic analysis

U2 - 10.1021/acsfoodscitech.1c00037

DO - 10.1021/acsfoodscitech.1c00037

M3 - RGC 21 - Publication in refereed journal

SN - 2692-1944

VL - 1

SP - 614

EP - 624

JO - ACS Food Science & Technology

JF - ACS Food Science & Technology

IS - 4

ER -

Synthetic Hexadecapeptide Prevents Postharvest Pectobacterium carotovorum (subsp. brasiliensis BC1) Infection via Destabilizing Cell Envelope and Suppressing Biosynthesis of Arginine and Peptidoglycan

Abstract

Research Keywords

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CRF: Development of Novel Inhibitors Targeting the Resistance Mechanisms of Clinical Superbugs

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