Synthesis and characterization of folate-PEG-grafted-hyperbranched-PEI for tumor-targeted gene delivery

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

125 Scopus Citations
View graph of relations

Author(s)

  • Bing Liang
  • Zhong-Peng Xiao
  • Yi Li
  • Chu-yan Chan
  • Hsiang-Fu Kung
  • Xin-Tao Shuai
  • Ying Peng

Detail(s)

Original languageEnglish
Pages (from-to)874-880
Journal / PublicationBiochemical and Biophysical Research Communications
Volume367
Issue number4
Publication statusPublished - 21 Mar 2008
Externally publishedYes

Abstract

A great challenge for gene therapy is to develop a high efficient gene delivery system with low toxicity. Nonviral vectors are still attractive although the current agents displayed some disadvantages (i.e., low transfection efficiency, high toxicity). To overcome the high toxicity of poly(ethylene imine) (PEI) and low transfection efficiency of PEGylated PEI (PEG-PEI), we linked a cell specific target molecule folate (FA) on poly(ethylene glycol) (PEG) and then grafted the FA-PEG onto hyperbranched PEI 25 kDa. The FA-PEG- grafted-hyperbranched-PEI (FA-PEG-PEI) effectively condensed plasmid DNA (pDNA) into nanoparticles with positive surface charge under a suitable N/P ratio. Tested in deferent cell lines (i.e., HEK 293T, glioma C6 and hepatoma HepG2 cells), no significant cytotoxicity of FA-PEG-PEI was added to PEG-PEI. More importantly, significant transfection efficiency was exhibited in FA-targeted cells. Reporter assay showed that FA-PEG-PEI/pDNA complexes had significantly higher transgene activity than that of PEI/pDNA in folate-receptor (FR) positive (HEK 293T and C6) cells but not FR-negative (HepG2) cells. These results indicated that FA-PEG-PEI might be a promising candidate for gene delivery with the characteristics of good biocompatibility, potential biodegradability, and relatively high gene transfection efficiency. © 2008 Elsevier Inc. All rights reserved.

Research Area(s)

  • FA-PEG-g-hy-PEI, FA-receptor, In vitro gene transfer, Transfection efficiency, Tumor targeting

Citation Format(s)

Synthesis and characterization of folate-PEG-grafted-hyperbranched-PEI for tumor-targeted gene delivery. / Liang, Bing; He, Ming-Liang; Xiao, Zhong-Peng; Li, Yi; Chan, Chu-yan; Kung, Hsiang-Fu; Shuai, Xin-Tao; Peng, Ying.

In: Biochemical and Biophysical Research Communications, Vol. 367, No. 4, 21.03.2008, p. 874-880.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review