Synergistic ex-situ biodegradation of crude oil by halotolerant bacterial consortium of indigenous strains isolated from on shore sites of Gujarat, India

Sunita J. Varjani; Dolly P. Rana; Ajay K. Jain; Surendra Bateja; Vivek N. Upasani

doi:10.1016/j.ibiod.2015.03.030

Synergistic ex-situ biodegradation of crude oil by halotolerant bacterial consortium of indigenous strains isolated from on shore sites of Gujarat, India

Sunita J. Varjani, Dolly P. Rana, Ajay K. Jain, Surendra Bateja, Vivek N. Upasani^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

233 Citations (Scopus)

Abstract

Hydrocarbon pollution due to activities of petroleum industry is a major problem worldwide especially in developing countries like India. Hydrocarbon utilizing bacterial consortium (HUBC) consisting of six (06) bacterial isolates viz. Ochrobactrum sp. (01), Stenotrophomonas maltophilia (02) and P. aeruginosa (03) isolated from crude oil polluted sites of ONGC fields of Gujarat, India. The isolates were identified by using VITEK®2 Compact and 16S rDNA gene sequencing. Scale up study of crude oil degradation (K#X, 3% v/v) by HUBC using Bushnell-Hass medium was performed under optimized growth conditions up to 75 days. Gravimetric and gas chromatographic analysis showed 83.70% and 83.49% (C8-C35) degradation, respectively. Presence of paraffins (C20-C40) in crude oil causes well clogging problem during exploration activities. The HUBC utilized paraffin content of crude oil and hence can be used to solve this problem. The findings of ex-situ bioaugmentation studies by halotolerant HUBC suggests its utility for effective in situ bioremediation of hydrocarbon contaminated sites as well as management of sea surface oil spills. © 2015 Elsevier Ltd.

Original language	English
Pages (from-to)	116-124
Number of pages	9
Journal	International Biodeterioration and Biodegradation
Volume	103
Online published	14 May 2015
DOIs	https://doi.org/10.1016/j.ibiod.2015.03.030
Publication status	Published - Sept 2015
Externally published	Yes

Research Keywords

16S rDNA
Biodegradation
Consortium
Ex-situ bioaugmentation
Gas chromatography
VITEK®2 Compact

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ibiod.2015.03.030

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title = "Synergistic ex-situ biodegradation of crude oil by halotolerant bacterial consortium of indigenous strains isolated from on shore sites of Gujarat, India",

abstract = "Hydrocarbon pollution due to activities of petroleum industry is a major problem worldwide especially in developing countries like India. Hydrocarbon utilizing bacterial consortium (HUBC) consisting of six (06) bacterial isolates viz. Ochrobactrum sp. (01), Stenotrophomonas maltophilia (02) and P. aeruginosa (03) isolated from crude oil polluted sites of ONGC fields of Gujarat, India. The isolates were identified by using VITEK{\textregistered}2 Compact and 16S rDNA gene sequencing. Scale up study of crude oil degradation (K#X, 3% v/v) by HUBC using Bushnell-Hass medium was performed under optimized growth conditions up to 75 days. Gravimetric and gas chromatographic analysis showed 83.70% and 83.49% (C8-C35) degradation, respectively. Presence of paraffins (C20-C40) in crude oil causes well clogging problem during exploration activities. The HUBC utilized paraffin content of crude oil and hence can be used to solve this problem. The findings of ex-situ bioaugmentation studies by halotolerant HUBC suggests its utility for effective in situ bioremediation of hydrocarbon contaminated sites as well as management of sea surface oil spills. {\textcopyright} 2015 Elsevier Ltd.",

keywords = "16S rDNA, Biodegradation, Consortium, Ex-situ bioaugmentation, Gas chromatography, VITEK{\textregistered}2 Compact",

author = "Varjani, {Sunita J.} and Rana, {Dolly P.} and Jain, {Ajay K.} and Surendra Bateja and Upasani, {Vivek N.}",

year = "2015",

month = sep,

doi = "10.1016/j.ibiod.2015.03.030",

language = "English",

volume = "103",

pages = "116--124",

journal = "International Biodeterioration and Biodegradation",

issn = "0964-8305",

publisher = "Elsevier Ltd.",

}

Synergistic ex-situ biodegradation of crude oil by halotolerant bacterial consortium of indigenous strains isolated from on shore sites of Gujarat, India. / Varjani, Sunita J.; Rana, Dolly P.; Jain, Ajay K. et al.
In: International Biodeterioration and Biodegradation, Vol. 103, 09.2015, p. 116-124.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

TY - JOUR

T1 - Synergistic ex-situ biodegradation of crude oil by halotolerant bacterial consortium of indigenous strains isolated from on shore sites of Gujarat, India

AU - Varjani, Sunita J.

AU - Rana, Dolly P.

AU - Jain, Ajay K.

AU - Bateja, Surendra

AU - Upasani, Vivek N.

PY - 2015/9

Y1 - 2015/9

N2 - Hydrocarbon pollution due to activities of petroleum industry is a major problem worldwide especially in developing countries like India. Hydrocarbon utilizing bacterial consortium (HUBC) consisting of six (06) bacterial isolates viz. Ochrobactrum sp. (01), Stenotrophomonas maltophilia (02) and P. aeruginosa (03) isolated from crude oil polluted sites of ONGC fields of Gujarat, India. The isolates were identified by using VITEK®2 Compact and 16S rDNA gene sequencing. Scale up study of crude oil degradation (K#X, 3% v/v) by HUBC using Bushnell-Hass medium was performed under optimized growth conditions up to 75 days. Gravimetric and gas chromatographic analysis showed 83.70% and 83.49% (C8-C35) degradation, respectively. Presence of paraffins (C20-C40) in crude oil causes well clogging problem during exploration activities. The HUBC utilized paraffin content of crude oil and hence can be used to solve this problem. The findings of ex-situ bioaugmentation studies by halotolerant HUBC suggests its utility for effective in situ bioremediation of hydrocarbon contaminated sites as well as management of sea surface oil spills. © 2015 Elsevier Ltd.

AB - Hydrocarbon pollution due to activities of petroleum industry is a major problem worldwide especially in developing countries like India. Hydrocarbon utilizing bacterial consortium (HUBC) consisting of six (06) bacterial isolates viz. Ochrobactrum sp. (01), Stenotrophomonas maltophilia (02) and P. aeruginosa (03) isolated from crude oil polluted sites of ONGC fields of Gujarat, India. The isolates were identified by using VITEK®2 Compact and 16S rDNA gene sequencing. Scale up study of crude oil degradation (K#X, 3% v/v) by HUBC using Bushnell-Hass medium was performed under optimized growth conditions up to 75 days. Gravimetric and gas chromatographic analysis showed 83.70% and 83.49% (C8-C35) degradation, respectively. Presence of paraffins (C20-C40) in crude oil causes well clogging problem during exploration activities. The HUBC utilized paraffin content of crude oil and hence can be used to solve this problem. The findings of ex-situ bioaugmentation studies by halotolerant HUBC suggests its utility for effective in situ bioremediation of hydrocarbon contaminated sites as well as management of sea surface oil spills. © 2015 Elsevier Ltd.

KW - 16S rDNA

KW - Biodegradation

KW - Consortium

KW - Ex-situ bioaugmentation

KW - Gas chromatography

KW - VITEK®2 Compact

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U2 - 10.1016/j.ibiod.2015.03.030

DO - 10.1016/j.ibiod.2015.03.030

M3 - RGC 21 - Publication in refereed journal

SN - 0964-8305

VL - 103

SP - 116

EP - 124

JO - International Biodeterioration and Biodegradation

JF - International Biodeterioration and Biodegradation

ER -

Synergistic ex-situ biodegradation of crude oil by halotolerant bacterial consortium of indigenous strains isolated from on shore sites of Gujarat, India

Abstract

Research Keywords

UN SDGs

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