Abstract
We developed a new integrated microfluidic chip to measure single cell secretions and sort cell without any harmful effects from sensing molecules. The parent droplet with single cell was split into two daughter droplets. Both daughter droplets were synchronized. One daughter droplet containing only supernatant was injected with sensors for assay; while another daughter droplet with single cell was sorted based on the assay result. By this method, cells were sorted without contacting any possible harmful effects from sensors.
| Original language | English |
|---|---|
| Title of host publication | 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2019) |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 24-25 |
| ISBN (Print) | 9781733419000, 9781713818526 |
| Publication status | Published - Oct 2019 |
| Externally published | Yes |
| Event | 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2019) - Congress Center Basel, Basel, Switzerland Duration: 27 Oct 2019 → 31 Oct 2019 https://cbmsociety.org/microtas/microtas2019/ |
Publication series
| Name | International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS |
|---|
Conference
| Conference | 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2019) |
|---|---|
| Abbreviated title | MicroTAS 2019 |
| Place | Switzerland |
| City | Basel |
| Period | 27/10/19 → 31/10/19 |
| Internet address |
Research Keywords
- Label-free
- Single cell secretion
- Sorting
- Synchronized droplet
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