Synaptotagmin IV regulates dense core vesicle (DCV) release in LβT2 cells

Zhi-Tao Hu; Mao-Rong Chen; Zhao Ping; Yong-Ming Dong; Rong-Ying Zhang; Tao Xu; Zheng-Xing Wu

doi:10.1016/j.bbrc.2008.04.174

Author(s)

Mao-Rong Chen
Zhao Ping
Yong-Ming Dong
Rong-Ying Zhang
Tao Xu
Zheng-Xing Wu

Detail(s)

Original language	English
Pages (from-to)	781-786
Journal / Publication	Biochemical and Biophysical Research Communications
Volume	371
Issue number	4
Publication status	Published - 11 Jul 2008
Externally published	Yes

Link(s)

DOI	DOI https://doi.org/10.1016/j.bbrc.2008.04.174 Final Published version
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Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-44349112878&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(c3516eaa-c810-40d4-8b18-35f085292c82).html

Abstract

Synaptotagmins (Syts) are calcium-binding proteins which are conserved from nematodes to humans. Fifteen Syts have been identified in mammalian species. Syt I is recognized as a Ca²⁺ sensor for the synchronized release of synaptic vesicles in some types of neurons, but its role in the secretion of dense core vesicles (DCVs) remains unclear. The function of Syt IV is of particular interest because it is rapidly up-regulated by chronic depolarization and seizures. Using RNAi-mediated gene silencing, we have explored the role of Syt I and IV on secretion in a pituitary gonadotrope cell line. Downregulation of Syt IV clearly reduced Ca²⁺-triggered exocytosis of dense core vesicles (DCVs) in LβT2 cells. Syt I silencing, however, had no effect on vesicular release. © 2008 Elsevier Inc. All rights reserved.

Research Area(s)

Ca2+ sensor, Double stranded RNA-mediated interference (RNAi), Exocytosis, LβT2 cell, Synaptotagmin

Bibliographic Note

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Citation Format(s)

[ RIS ] [ BibTeX ]

Synaptotagmin IV regulates dense core vesicle (DCV) release in LβT2 cells. / Hu, Zhi-Tao; Chen, Mao-Rong; Ping, Zhao et al.
In: Biochemical and Biophysical Research Communications, Vol. 371, No. 4, 11.07.2008, p. 781-786.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

Hu, Z-T, Chen, M-R, Ping, Z, Dong, Y-M, Zhang, R-Y, Xu, T & Wu, Z-X 2008, 'Synaptotagmin IV regulates dense core vesicle (DCV) release in LβT2 cells', Biochemical and Biophysical Research Communications, vol. 371, no. 4, pp. 781-786. https://doi.org/10.1016/j.bbrc.2008.04.174

Hu, Z.-T., Chen, M.-R., Ping, Z., Dong, Y.-M., Zhang, R.-Y., Xu, T., & Wu, Z.-X. (2008). Synaptotagmin IV regulates dense core vesicle (DCV) release in LβT2 cells. Biochemical and Biophysical Research Communications, 371(4), 781-786. https://doi.org/10.1016/j.bbrc.2008.04.174

Hu ZT, Chen MR, Ping Z, Dong YM, Zhang RY, Xu T et al. Synaptotagmin IV regulates dense core vesicle (DCV) release in LβT2 cells. Biochemical and Biophysical Research Communications. 2008 Jul 11;371(4):781-786. doi: 10.1016/j.bbrc.2008.04.174

Hu, Zhi-Tao ; Chen, Mao-Rong ; Ping, Zhao et al. / Synaptotagmin IV regulates dense core vesicle (DCV) release in LβT2 cells. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 371, No. 4. pp. 781-786.