Suppressed primary osteoblast functions on nanoporous titania surface

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Lingzhou Zhao
  • Shenglin Mei
  • Wei Wang
  • Yumei Zhang
  • Zhifen Wu

Detail(s)

Original languageEnglish
Pages (from-to)100-107
Journal / PublicationJournal of Biomedical Materials Research - Part A
Volume96 A
Issue number1
Publication statusPublished - Jan 2011

Abstract

Titiania nanotubes have large potential in medical implant applications but their tissue compatibility is still controversial. Considering that the biological behavior of primary osteoblasts is closer to the in vivo situation than other common cell lines, we investigate the response of primary osteoblasts on anodized nanotextured titania surfaces. Two nanotextured surface morphologies, namely the 5 V anodized surface with a pore diameter of 25 nm and the 20 V anodized surface with a tube diameter of 80 nm are chosen for this study. Initial cell adhesion is not obviously affected by the anodized surfaces. With the exception of slightly higher intracellular alkaline phosphatase activity and more extracellular matrix deposition, cell growth, and cell differentiation represented by the expressions of osteogenesis-related genes are impaired on both anodized surfaces. This may be attributed to the compromised focal contact formation on the anodized surfaces. The difference in the phenotypes of the primary osteoblasts and the osteoblastic cell lines may partly account for the controversy in osteoblast cytocompatibility on titania nanotubes. © 2010 Wiley Periodicals, Inc.

Research Area(s)

  • alkaline phosphatase activity, cell adhesion, nanotopography, primary osteoblast, real-time PCR

Citation Format(s)

Suppressed primary osteoblast functions on nanoporous titania surface. / Zhao, Lingzhou; Mei, Shenglin; Wang, Wei; Chu, Paul K.; Zhang, Yumei; Wu, Zhifen.

In: Journal of Biomedical Materials Research - Part A, Vol. 96 A, No. 1, 01.2011, p. 100-107.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review