Super-Resolution Photoacoustic Microscopy Using Structured-Illumination

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

10 Scopus Citations
View graph of relations


  • Mohammadreza Amjadian
  • Seyed Masood Mostafavi
  • Zahra Kavehvash

Related Research Unit(s)


Original languageEnglish
Pages (from-to)2197-2207
Number of pages11
Journal / PublicationIEEE Transactions on Medical Imaging
Issue number9
Online published15 Apr 2021
Publication statusPublished - Sept 2021


A novel super-resolution volumetric photoacoustic microscopy, based on the theory of structured-illumination, is proposed in this paper. The structured-illumination will be introduced in order to surpass the diffraction limit in a photoacoustic microscopy (PAM) structure. Through optical excitation of the targeted object with a sinusoidal spatial fringe pattern, the object’s frequency spectrum is forced to shift in the spatial frequency domain. The shifting in the desired direction leads to the passage of the high-frequency contents of the object through the passband of the acoustic diffraction frequency response. Finally, combining the low-frequency image with the high-frequency parts in four regular orientations in the spatial frequency domain is equivalent to imaging the targeted object with an imaging system of two-fold bandwidth and thus half lateral resolution. In order to obtain the image of out-of-focus regions and improve the lateral resolution outside the focal region of a PAM imaging system, Fourier-domain reconstruction algorithm based on the synthetic aperture focusing technique (SAFT) using the virtual detector concept is utilized for reduction in the required computational load and time. The performance of the proposed imaging system is validated with in vivo and ex vivo targets. The experimental results obtained from several tungsten filaments in the depth range of 1.2 mm, show an improvement of -6 dB lateral resolution from 55-287 μm to 25-29 μm and also an improvement of signal-to-noise ratio (SNR) from 16-22 dB to 27-33 dB in the proposed system.

Research Area(s)

  • Focusing, Fourier-domain reconstruction, Image reconstruction, Image resolution, Imaging, Lighting, Microscopy, Speckle, structured-illumination, super-resolution, synthetic aperture focusing technique, virtual detector, Volumetric photoacoustic microscopy

Citation Format(s)

Super-Resolution Photoacoustic Microscopy Using Structured-Illumination. / Amjadian, Mohammadreza; Mostafavi, Seyed Masood; Chen, Jiangbo et al.
In: IEEE Transactions on Medical Imaging, Vol. 40, No. 9, 09.2021, p. 2197-2207.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review