@inproceedings{ceb1dbb89ad74dc3895ab60e784845f0,
title = "Superoxide detection by chemiluminescence, fluorescence, and spectrophotometry methods",
abstract = "Pathology of vessel wall endothelium has been attributed to the presence of reactive oxygen species (ROS) and its effects during the onset of oxidative stress. The root of all ROS produced is superoxide (O2•-). Hence, its detection is imperative in filtering out appropriate antioxidants and obtaining deeper knowledge on its role. The present study used lucigenin-enhanced chemiluminescence, hydroethidine fluorescence, and cytochrome c spectrophotometric assays to measure O2•-. The aim was to compare and contrast among the methods using human embryonic kidney cells (HEK). Superoxide production was induced by NADH via NAD(P)H oxidase system. The study showed that lucigenin enhanced chemiluminescence assay was the most sensitive method whilst hydroethidine and cytochrome c assays were proficient as well. {\textcopyright} 2006 Research Publishing Services.",
keywords = "Cytochrome c, Free radicals, Hydroethidine, Lucigenin, Superoxide",
author = "Susanto, \{Anindya P.\} and Mohammad Firdhaosh and Dong-An Wang and Moe, \{Kyaw Thu\}",
year = "2006",
month = dec,
language = "English",
isbn = "8190426249",
series = "ICBPE - Proceedings of the International Conference on Biomedical and Pharmaceutical Engineering",
publisher = "IEEE",
pages = "537--540",
booktitle = "ICBPE 2006 - Proceedings of the 2006 International Conference on Biomedical and Pharmaceutical Engineering",
address = "United States",
note = "2006 International Conference on Biomedical and Pharmaceutical Engineering (ICBPE 2006), ICBPE2006 ; Conference date: 11-12-2006 Through 14-12-2006",
}