Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging

Amnon Bar-Shir; Yajie Liang; Kannie W. Y. Chan; Assaf A. Gilad; Jeff W. M. Bulte

doi:10.1039/c4cc10195b

Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging

Amnon Bar-Shir
, Yajie Liang
, Kannie W. Y. Chan
, Assaf A. Gilad
, Jeff W. M. Bulte^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

38 Citations (Scopus)

Abstract

Superpositively charged mutants of green fluorescent protein (GFP) demonstrated a dramatically improved chemical exchange saturation transfer (CEST) MRI contrast compared to their wild type counterparts. The mutants +36 GFP and +48 GFP were successfully expressed in mammalian cells and retained part of their fluorescence, making them a new potential bimodal reporter gene.

Original language	English
Pages (from-to)	4869-4871
Journal	Chemical Communications
Volume	51
Issue number	23
DOIs	https://doi.org/10.1039/c4cc10195b
Publication status	Published - 21 Mar 2015
Externally published	Yes

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title = "Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging",

abstract = "Superpositively charged mutants of green fluorescent protein (GFP) demonstrated a dramatically improved chemical exchange saturation transfer (CEST) MRI contrast compared to their wild type counterparts. The mutants +36 GFP and +48 GFP were successfully expressed in mammalian cells and retained part of their fluorescence, making them a new potential bimodal reporter gene.",

author = "Amnon Bar-Shir and Yajie Liang and Chan, \{Kannie W. Y.\} and Gilad, \{Assaf A.\} and Bulte, \{Jeff W. M.\}",

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doi = "10.1039/c4cc10195b",

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T1 - Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging

AU - Bar-Shir, Amnon

AU - Liang, Yajie

AU - Chan, Kannie W. Y.

AU - Gilad, Assaf A.

AU - Bulte, Jeff W. M.

PY - 2015/3/21

Y1 - 2015/3/21

N2 - Superpositively charged mutants of green fluorescent protein (GFP) demonstrated a dramatically improved chemical exchange saturation transfer (CEST) MRI contrast compared to their wild type counterparts. The mutants +36 GFP and +48 GFP were successfully expressed in mammalian cells and retained part of their fluorescence, making them a new potential bimodal reporter gene.

AB - Superpositively charged mutants of green fluorescent protein (GFP) demonstrated a dramatically improved chemical exchange saturation transfer (CEST) MRI contrast compared to their wild type counterparts. The mutants +36 GFP and +48 GFP were successfully expressed in mammalian cells and retained part of their fluorescence, making them a new potential bimodal reporter gene.

UR - https://www.scopus.com/pages/publications/84924368523

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84924368523&origin=recordpage

U2 - 10.1039/c4cc10195b

DO - 10.1039/c4cc10195b

M3 - RGC 21 - Publication in refereed journal

C2 - 25697683

SN - 0009-241X

VL - 51

SP - 4869

EP - 4871

JO - Chemical Communications

JF - Chemical Communications

IS - 23

ER -

Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging

Abstract

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