Subcellular localization of GABAB receptor subunits in rat globus pallidus

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

Detail(s)

Original languageEnglish
Pages (from-to)340-352
Journal / PublicationJournal of Comparative Neurology
Volume474
Issue number3
Publication statusPublished - 28 Jun 2004
Externally publishedYes

Abstract

The inhibitory amino acid γ-aminobutyric acid (GABA) is the major neurotransmitter in the globus pallidus. Although electrophysiological studies have indicated that functional GABAB receptors exist in rat globus pallidus, the subcellular localization of GABAB receptor subunits and their spatial relationship to glutamatergic and GABAergic synapses are unknown. Here, we use pre-embedding immunogold labeling to study the subcellular localization of GABAB receptor subunits, GABA B1 and GABAB2, in globus pallidus neurons and identified populations of afferent terminals. Immunolabeling for GABAB1 and GABAB2 was observed throughout the globus pallidus, with GABA B1 more strongly expressed in perikarya and GABAB2 mainly expressed in the neuropil. Electron microscopic analysis revealed that the majority of GABAB1 labeling was localized within the cytoplasm, whereas most of GABAB2 labeling was associated with the plasma membrane. At the subcellular level, both the GABAB1 and GABA B2 immunogold labeling was localized at pre- and postsynaptic sites. At asymmetric, putative excitatory, synapses, GABAB1 and GABA B2 immunogold labeling was found at perisynaptic sites of both pre- and postsynaptic specializations. Double immunolabeling, using the vesicular glutamate transporter 2 (VGLUT2), revealed the glutamatergic nature of most immunogold-labeled asymmetric synapses. At symmetric, putative GABAergic, synapses, including those formed by anterogradely labeled striatopallidal terminals, GABAB1 and GABAB2 immunogold labeling was found in the main body of both pre- and postsynaptic specializations. These results demonstrate the existence of presynaptic GABAB auto- and heteroreceptors and postsynaptic GABAB receptors, which may be involved in modulating synaptic transmission in the globus pallidus. © 2004 Wiley-Liss, Inc.

Research Area(s)

  • Basal ganglia, Pre-embedding immunogold, Synapses, striatopallidal, VGLUT2

Bibliographic Note

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Citation Format(s)

Subcellular localization of GABAB receptor subunits in rat globus pallidus. / Chen, Lei; Boyes, Justin; Yung, Wing-Ho et al.
In: Journal of Comparative Neurology, Vol. 474, No. 3, 28.06.2004, p. 340-352.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review