TY - GEN
T1 - Study on Theileria lestoquardi Antigens as Potential Vaccine Candidates
AU - Namavari, Mehdi
AU - Hosseini, Mohammad H.
AU - Seghatoleslam, Atefeh
AU - Lotfi, Mohsen
AU - Shirazi, Ali
AU - Sparagano, Olivier A. E.
PY - 2008/12
Y1 - 2008/12
N2 - Theileria lestoquardi is the causative agent of malignant theileriosis of sheep and goats, causing morbidity and mortality in these animals worldwide. Western blot analysis based on T. lestoquardi schizont antigens was carried out using sera collected from Iranian sheep, which had been immunized with T. lestoquardi schizont-infected cells. The results of Western blot analysis demonstrated that schizont-immunized animals produced antibodies reacting with protein bands at 73, 42, 20, 14, and 12 kDa. Comparison of the results of the current Western blotting test with earlier studies of Theileria spp. revealed two immunogenic schizont proteins with molecular weights of 73 and 42 kDa shared between T. annulata and T. lestoquardi. Two other proteins with molecular weights of 14 and 12 kDa have not been previously found in other Theileria species. Our results suggest that the 73-kDa protein could be a potential vaccine candidate and that the 14- and 12-kDa proteins could be considered as diagnostic antigens.
AB - Theileria lestoquardi is the causative agent of malignant theileriosis of sheep and goats, causing morbidity and mortality in these animals worldwide. Western blot analysis based on T. lestoquardi schizont antigens was carried out using sera collected from Iranian sheep, which had been immunized with T. lestoquardi schizont-infected cells. The results of Western blot analysis demonstrated that schizont-immunized animals produced antibodies reacting with protein bands at 73, 42, 20, 14, and 12 kDa. Comparison of the results of the current Western blotting test with earlier studies of Theileria spp. revealed two immunogenic schizont proteins with molecular weights of 73 and 42 kDa shared between T. annulata and T. lestoquardi. Two other proteins with molecular weights of 14 and 12 kDa have not been previously found in other Theileria species. Our results suggest that the 73-kDa protein could be a potential vaccine candidate and that the 14- and 12-kDa proteins could be considered as diagnostic antigens.
KW - Antigen
KW - Schizont
KW - Sheep
KW - Theileria lestoquardi
UR - https://www.scopus.com/pages/publications/57849131924
UR - https://www.scopus.com/pages/publications/57849131924#tab=impact
U2 - 10.1196/annals.1428.020
DO - 10.1196/annals.1428.020
M3 - RGC 32 - Refereed conference paper (with host publication)
C2 - 19120211
SN - 9781573317146
T3 - Annals of the New York Academy of Sciences
SP - 205
EP - 207
BT - Animal Biodiversity and Emerging Diseases Prediction and Prevention
PB - Blackwell Publishing Inc.
T2 - 9th Biennial Conference of the International Society for Tropical Veterinary Medicine (STVM 2007)
Y2 - 17 June 2007 through 22 June 2007
ER -