Study of the Aggregation of DNA-Capped Gold Nanoparticles: A Smart and Flexible Aptasensor for Spermine Sensing

Tik-Hung Tsoi; Yan-Juan Gu; Wai-Sum Lo; Wai-Ting Wong; Wing-Tak Wong; Chi-Fai Ng; Chi-Sing Lee; Ka-Leung Wong

doi:10.1002/cplu.201700155

Study of the Aggregation of DNA-Capped Gold Nanoparticles: A Smart and Flexible Aptasensor for Spermine Sensing

Tik-Hung Tsoi
, Yan-Juan Gu
, Wai-Sum Lo
, Wai-Ting Wong
, Wing-Tak Wong
, Chi-Fai Ng
, Chi-Sing Lee
, Ka-Leung Wong

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

18 Citations (Scopus)

Abstract

A smart gold nanoparticle based aptasensor is developed for the sensing of this biomarker in a convenient and fast manner. A comprehensive study was performed to elucidate the driving force of DNA adsorption, different factors’ effects, such as gold nanoparticle size, DNA length, concentration, and working pH towards spermine sensing by using UV/Vis absorption spectroscopy and isothermal titration calorimetry. It was found that the developed aptasensor could detect spermine by two different sensing mechanisms simply by adjusting the DNA concentration without complicated procedures. Good performance in complicated matrices was proven by the satisfactory results obtained in the spike analysis of both artificial urine and clinical urine samples. Such a flexible and smart approach described here would provide a useful tool for the fast sensing of spermine and prostate cancer screening. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

Original language	English
Pages (from-to)	802-809
Journal	ChemPlusChem
Volume	82
Issue number	5
DOIs	https://doi.org/10.1002/cplu.201700155
Publication status	Published - 1 May 2017
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Funding

The project was funded by the University Research Facility for Chemical and Environmental Analysis (UCEA), and Area of Excellent Grants (1-ZVGG) of The Hong Kong Polytechnic University, Matching Proof-of-Concept Fund of Hong Kong Baptist University (MPCF-05-2016/17), and Shenzhen Science and Technology Innovation Committee (JCYJ20160330095659560 and JCYJ20150626111042525). The authors declare no competing financial interest.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Keywords

DNA
gold
nanoparticles
spermine
urine

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abstract = "A smart gold nanoparticle based aptasensor is developed for the sensing of this biomarker in a convenient and fast manner. A comprehensive study was performed to elucidate the driving force of DNA adsorption, different factors{\textquoteright} effects, such as gold nanoparticle size, DNA length, concentration, and working pH towards spermine sensing by using UV/Vis absorption spectroscopy and isothermal titration calorimetry. It was found that the developed aptasensor could detect spermine by two different sensing mechanisms simply by adjusting the DNA concentration without complicated procedures. Good performance in complicated matrices was proven by the satisfactory results obtained in the spike analysis of both artificial urine and clinical urine samples. Such a flexible and smart approach described here would provide a useful tool for the fast sensing of spermine and prostate cancer screening. {\textcopyright} 2017 Wiley-VCH Verlag GmbH \& Co. KGaA, Weinheim",

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AU - Wong, Wai-Ting

AU - Wong, Wing-Tak

AU - Ng, Chi-Fai

AU - Lee, Chi-Sing

AU - Wong, Ka-Leung

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AB - A smart gold nanoparticle based aptasensor is developed for the sensing of this biomarker in a convenient and fast manner. A comprehensive study was performed to elucidate the driving force of DNA adsorption, different factors’ effects, such as gold nanoparticle size, DNA length, concentration, and working pH towards spermine sensing by using UV/Vis absorption spectroscopy and isothermal titration calorimetry. It was found that the developed aptasensor could detect spermine by two different sensing mechanisms simply by adjusting the DNA concentration without complicated procedures. Good performance in complicated matrices was proven by the satisfactory results obtained in the spike analysis of both artificial urine and clinical urine samples. Such a flexible and smart approach described here would provide a useful tool for the fast sensing of spermine and prostate cancer screening. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

KW - DNA

KW - gold

KW - nanoparticles

KW - spermine

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Study of the Aggregation of DNA-Capped Gold Nanoparticles: A Smart and Flexible Aptasensor for Spermine Sensing

Abstract

Bibliographical note

Funding

UN SDGs

Research Keywords

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