Structural microgels for biological binding assays via droplet microfluidics

M. N. Hsu; E. X. Ng; Y. Zhang; C. H. Chen

Structural microgels for biological binding assays via droplet microfluidics

M. N. Hsu
, E. X. Ng
, Y. Zhang
, C. H. Chen^*

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

Abstract

As advances in medicine are increasingly dependent on the understanding of multiple molecular etiologies of diseases, development of ultra-sensitive and multiplexed biomarker detection assays becomes crucial. Hydrogel particles, due to their porous nature, offer multiple advantages over traditional bead assays. However, the sensitivity of hydrogel particle assays remains low at pM range. Hence, this work reports a novel sensitivity enhancement platform through the use of temperature responsive hydrogel particles fabricated via droplet microfluidics. The temperature induced shrinkage of hydrogel particles allows the concentration of analytes within the gel matrix, enabling instant signal amplification without the need for the time consuming enzyme-substrate approach. © (2016) by Chemical and Biological Microsystems Society

Original language	English
Title of host publication	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)
Publisher	Chemical and Biological Microsystems Society
Pages	1246-1247
ISBN (Print)	9780979806490, 978-1-5108-3416-3
Publication status	Published - Oct 2016
Externally published	Yes
Event	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016) - Convention Center Dublin, Dublin, Ireland Duration: 9 Oct 2016 → 13 Oct 2016 http://docplayer.net/51234665-Conference-at-a-glance.html http://www.microtas2016.org/

Publication series

Name	International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS

Conference

Conference	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)
Abbreviated title	MicroTAS 2016
Place	Ireland
City	Dublin
Period	9/10/16 → 13/10/16
Internet address	http://docplayer.net/51234665-Conference-at-a-glance.html http://www.microtas2016.org/

Research Keywords

Hydrogel particles
Immunoassay
Microfluidic process
Sensitivity enhancement

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Hsu, M. N., Ng, E. X., Zhang, Y., & Chen, C. H. (2016). Structural microgels for biological binding assays via droplet microfluidics. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016) (pp. 1246-1247). (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS). Chemical and Biological Microsystems Society.

Hsu, M. N. ; Ng, E. X. ; Zhang, Y. et al. / Structural microgels for biological binding assays via droplet microfluidics. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016). Chemical and Biological Microsystems Society, 2016. pp. 1246-1247 (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS).

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title = "Structural microgels for biological binding assays via droplet microfluidics",

abstract = "As advances in medicine are increasingly dependent on the understanding of multiple molecular etiologies of diseases, development of ultra-sensitive and multiplexed biomarker detection assays becomes crucial. Hydrogel particles, due to their porous nature, offer multiple advantages over traditional bead assays. However, the sensitivity of hydrogel particle assays remains low at pM range. Hence, this work reports a novel sensitivity enhancement platform through the use of temperature responsive hydrogel particles fabricated via droplet microfluidics. The temperature induced shrinkage of hydrogel particles allows the concentration of analytes within the gel matrix, enabling instant signal amplification without the need for the time consuming enzyme-substrate approach. {\textcopyright} (2016) by Chemical and Biological Microsystems Society",

keywords = "Hydrogel particles, Immunoassay, Microfluidic process, Sensitivity enhancement",

author = "Hsu, \{M. N.\} and Ng, \{E. X.\} and Y. Zhang and Chen, \{C. H.\}",

year = "2016",

month = oct,

language = "English",

isbn = "9780979806490",

series = "International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS",

publisher = "Chemical and Biological Microsystems Society",

pages = "1246--1247",

booktitle = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)",

note = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016), MicroTAS 2016 ; Conference date: 09-10-2016 Through 13-10-2016",

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Hsu, MN, Ng, EX, Zhang, Y & Chen, CH 2016, Structural microgels for biological binding assays via droplet microfluidics. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016). International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS, Chemical and Biological Microsystems Society, pp. 1246-1247, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016), Dublin, Ireland, 9/10/16.

Structural microgels for biological binding assays via droplet microfluidics. / Hsu, M. N.; Ng, E. X.; Zhang, Y. et al.
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016). Chemical and Biological Microsystems Society, 2016. p. 1246-1247 (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - Structural microgels for biological binding assays via droplet microfluidics

AU - Hsu, M. N.

AU - Ng, E. X.

AU - Zhang, Y.

AU - Chen, C. H.

PY - 2016/10

Y1 - 2016/10

N2 - As advances in medicine are increasingly dependent on the understanding of multiple molecular etiologies of diseases, development of ultra-sensitive and multiplexed biomarker detection assays becomes crucial. Hydrogel particles, due to their porous nature, offer multiple advantages over traditional bead assays. However, the sensitivity of hydrogel particle assays remains low at pM range. Hence, this work reports a novel sensitivity enhancement platform through the use of temperature responsive hydrogel particles fabricated via droplet microfluidics. The temperature induced shrinkage of hydrogel particles allows the concentration of analytes within the gel matrix, enabling instant signal amplification without the need for the time consuming enzyme-substrate approach. © (2016) by Chemical and Biological Microsystems Society

AB - As advances in medicine are increasingly dependent on the understanding of multiple molecular etiologies of diseases, development of ultra-sensitive and multiplexed biomarker detection assays becomes crucial. Hydrogel particles, due to their porous nature, offer multiple advantages over traditional bead assays. However, the sensitivity of hydrogel particle assays remains low at pM range. Hence, this work reports a novel sensitivity enhancement platform through the use of temperature responsive hydrogel particles fabricated via droplet microfluidics. The temperature induced shrinkage of hydrogel particles allows the concentration of analytes within the gel matrix, enabling instant signal amplification without the need for the time consuming enzyme-substrate approach. © (2016) by Chemical and Biological Microsystems Society

KW - Hydrogel particles

KW - Immunoassay

KW - Microfluidic process

KW - Sensitivity enhancement

UR - https://www.scopus.com/pages/publications/85014216223

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85014216223&origin=recordpage

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9780979806490

SN - 978-1-5108-3416-3

T3 - International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS

SP - 1246

EP - 1247

BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)

PB - Chemical and Biological Microsystems Society

T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)

Y2 - 9 October 2016 through 13 October 2016

ER -

Hsu MN, Ng EX, Zhang Y , Chen CH. Structural microgels for biological binding assays via droplet microfluidics. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016). Chemical and Biological Microsystems Society. 2016. p. 1246-1247. (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS).

Structural microgels for biological binding assays via droplet microfluidics

Abstract

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Research Keywords

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