TY - JOUR
T1 - Structural diversity and antioxidant activity of condensed tannins fractionated from mangosteen pericarp
AU - Zhou, Hai-Chao
AU - Lin, Yi-Ming
AU - Wei, Shu-Dong
AU - Tam, Nora Fung-Yee
PY - 2011/12/15
Y1 - 2011/12/15
N2 - The extract from mangosteen pericarp was separated into eight fractions, four by solvent extraction (hexane, FH; petroleum ether, FP; ethyl acetate, FE; water, FW) and the other four by fractionation on Sephadex LH-20 column (fractions F1, F2, F3, and F4). Phenolic compounds were identified as the major antioxidant components in each fraction. UV-VIS spectrum, reversed-phase HPLC-ESI-MS coupled with thiolysis, normal-phase HPLC-ESI-MS and MALDI-TOF-MS analyses complementally showed that the phenolic-rich fractions were condensed tannins with structural heterogeneity in monomer units, degree of polymerization and interflavan linkages. Mangosteen condensed tannins predominately contained procyanidins with a significant amount of propelargonidin but much lower signals of prodelphinidin. Eicosapentamers of condensed tannins were detected by MALDI-TOF-MS. Both B-type and A-type linkages were present. Condensed tannins fractions from mangosteen pericarp, especially fractions F3 and FE, can be explored as beneficial food antioxidants because of their high yields and potent antioxidant activities. © 2011 Elsevier Ltd. All rights reserved.
AB - The extract from mangosteen pericarp was separated into eight fractions, four by solvent extraction (hexane, FH; petroleum ether, FP; ethyl acetate, FE; water, FW) and the other four by fractionation on Sephadex LH-20 column (fractions F1, F2, F3, and F4). Phenolic compounds were identified as the major antioxidant components in each fraction. UV-VIS spectrum, reversed-phase HPLC-ESI-MS coupled with thiolysis, normal-phase HPLC-ESI-MS and MALDI-TOF-MS analyses complementally showed that the phenolic-rich fractions were condensed tannins with structural heterogeneity in monomer units, degree of polymerization and interflavan linkages. Mangosteen condensed tannins predominately contained procyanidins with a significant amount of propelargonidin but much lower signals of prodelphinidin. Eicosapentamers of condensed tannins were detected by MALDI-TOF-MS. Both B-type and A-type linkages were present. Condensed tannins fractions from mangosteen pericarp, especially fractions F3 and FE, can be explored as beneficial food antioxidants because of their high yields and potent antioxidant activities. © 2011 Elsevier Ltd. All rights reserved.
KW - Antioxidant activity
KW - Condensed tannins
KW - Degree of polymerization
KW - Fractionation
KW - Mangosteen pericarp
KW - Structure-activity
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U2 - 10.1016/j.foodchem.2011.06.036
DO - 10.1016/j.foodchem.2011.06.036
M3 - RGC 21 - Publication in refereed journal
SN - 0308-8146
VL - 129
SP - 1710
EP - 1720
JO - Food Chemistry
JF - Food Chemistry
IS - 4
ER -