Strain-Promoted Alkyne-Azide Cycloaddition (SPAAC)-Based Bioorthogonal Labeling, Bioimaging, and Photocytotoxicity Studies of Phosphorescent Ruthenium(II) Polypyridine Dibenzocyclooctyne (DIBO) Complexes

Research output: Conference Papers (RGC: 31A, 31B, 32, 33)33_Other conference paper

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Author(s)

  • Kenneth Yin Zhang
  • Hua-Wei Liu
  • Po Lam Wu
  • King Fai Li
  • Kok Wai Cheah

Detail(s)

Original languageEnglish
Publication statusPresented - 18 Apr 2015

Conference

Title22nd Symposium on Chemistry Postgraduate Research in Hong Kong
PlaceChina
CityHong Kong
Period18 April 2015

Abstract

Four new ruthenium(II) polypyridine complexes functionalized with a dibenzocyclooctyne (DIBO) or amine moiety, [Ru(N^N)2(L)](PF6)2 (N^N = bpy, phen; L = bpy-DIBO, bpy-NH2) have been synthesized. The electronic absorption, photophysical properties, and lipophilicity of these complexes have been studied. The azide-reactive DIBO complexes have been used as bioorthogonal probes for the cell-surface N-azidoglycan units of Ac4ManNAz-pretreated cells through the SPAAC reaction. The live cell labeling properties of the DIBO complexes have been examined by laser-scanning confocal microscopy, flow cytometry, and ICP-MS.

Citation Format(s)

Strain-Promoted Alkyne-Azide Cycloaddition (SPAAC)-Based Bioorthogonal Labeling, Bioimaging, and Photocytotoxicity Studies of Phosphorescent Ruthenium(II) Polypyridine Dibenzocyclooctyne (DIBO) Complexes. / TANG, Tommy Siu-Ming; Yip, Alex Man-Hei; Zhang, Kenneth Yin; Liu, Hua-Wei; Wu, Po Lam; Li, King Fai; Cheah, Kok Wai; Lo, Kenneth Kam-Wing.

2015. 22nd Symposium on Chemistry Postgraduate Research in Hong Kong, Hong Kong, China.

Research output: Conference Papers (RGC: 31A, 31B, 32, 33)33_Other conference paper