Stable gold nanoparticle conjugation to internal DNA positions : Facile generation of discrete gold nanoparticle-DNA assemblies

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • Yongqiang Wen
  • Christopher K. McLaughlin
  • Pik Kwan Lo
  • Hua Yang
  • Hanadi F. Sleiman

Detail(s)

Original languageEnglish
Pages (from-to)1413-1416
Journal / PublicationBioconjugate Chemistry
Volume21
Issue number8
Publication statusPublished - 18 Aug 2010
Externally publishedYes

Abstract

A straightforward strategy to stably anchor one or more gold nanoparticles (AuNPs) at both the internal and terminal positions of single-stranded DNA is presented. Discrete DNA-AuNP conjugates are isolated using a noncovalent extension strand strategy, that helps to resolve prepared species by agarose gel electrophoresis (AGE). These are used to assemble well-defined AuNP squares and rectangles. Two complementary bis-AuNP-labeled DNA conjugates are then prepared. One of these places two smaller (5 nm) AuNPs at defined internal positions within a DNA strand, and the other places two larger (13 nm) AuNPs at each of its terminal positions. We show the self-assembly of these bis-AuNP conjugates into a tapered tetrameric gold nanoparticle "antenna" structures of direct relevance to engineered "hot spots" and surface enhanced Raman scattering (SERS) substrates. © 2010 American Chemical Society.

Citation Format(s)

Stable gold nanoparticle conjugation to internal DNA positions : Facile generation of discrete gold nanoparticle-DNA assemblies. / Wen, Yongqiang; McLaughlin, Christopher K.; Lo, Pik Kwan; Yang, Hua; Sleiman, Hanadi F.

In: Bioconjugate Chemistry, Vol. 21, No. 8, 18.08.2010, p. 1413-1416.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal