Stabilized albumin coatings on engineered xenografts for attenuation of acute immune and inflammatory responses
Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review
Author(s)
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Detail(s)
Original language | English |
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Pages (from-to) | 6080-6091 |
Journal / Publication | Journal of Materials Chemistry B |
Volume | 8 |
Issue number | 28 |
Online published | 18 Jun 2020 |
Publication status | Published - 28 Jul 2020 |
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Abstract
Xenogeneic grafts are promising candidates for transplantation therapy due to their easily accessible sources. Nevertheless, the immune and inflammatory responses induced by xenografts need to be addressed for clinical use. A novel and facile method was introduced for the attenuation of immune and inflammatory responses by extending the immune evasion potential of albumin to the tissue engineering field and coating albumin, which could passivate biomaterial surfaces, onto xenografts. Albumin was first modified by dopamine to enhance its adhesion on graft surfaces. Porcine chondrocytes derived living hyaline cartilage graft (LhCG) and decellularized LhCG (dLhCG) were applied as xenograft models implanted in the omentum of rats. Both LhCG which contained porcine chondrocytes as well as secreted ECM and dLhCG which was mainly composed of the porcine source ECM showed alleviated immune and inflammatory responses after being coated with albumin at cell, protein and gene levels, respectively. Significantly less inflammatory cells including neutrophils, macrophages and lymphocytes were recruited according to pathological analysis and immunohistochemistry staining with lower gene expression encoding inflammation-related cytokines including MCP-1, IL-6 and IL-1β after employing LhCG and dLhCG with albumin passivation coating.
Bibliographic Note
Full text of this publication does not contain sufficient affiliation information. With consent from the author(s) concerned, the Research Unit(s) information for this record is based on the existing academic department affiliation of the author(s).
Citation Format(s)
Stabilized albumin coatings on engineered xenografts for attenuation of acute immune and inflammatory responses. / Tao, Chao; Zhu, Wenzhen; Iqbal, Jabed et al.
In: Journal of Materials Chemistry B, Vol. 8, No. 28, 28.07.2020, p. 6080-6091 .
In: Journal of Materials Chemistry B, Vol. 8, No. 28, 28.07.2020, p. 6080-6091 .
Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review