Spectroscopic examination of enamel and dentin indicates erosion and staining by coffee through sequestration of elements

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review

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Detail(s)

Original languageEnglish
Title of host publicationProceedings of SPIE
Subtitle of host publicationLasers in Dentistry XXV
EditorsPeter Rechmann, Daniel Fried
PublisherSPIE
Volume10857
ISBN (Electronic)9781510623576
ISBN (Print)9781510623569
Publication statusPublished - Feb 2019

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10857
ISSN (Print)1605-7422

Conference

TitleLasers in Dentistry XXV 2019
PlaceUnited States
CitySan Francisco
Period2 - 7 February 2019

Abstract

The mechanism of coffee eliciting erosion on teeth is unclear as few studies have investigated the direct effect of coffee on enamel and dentin structures. The present study identified how coffee, the most popular beverage worldwide, induces staining and erosion on teeth. We show the grade of erosion of molars and incisors in Sprague Dawley rats from two different age groups, young (four weeks) and old (six months). We quantified the concentration of metals contained in coffee by mass spectrometry (ICP-MS). To determine elemental content in enamel (i.e. superficial) and dentin (i.e. substructure), we used Laser-induced Breakdown Spectroscopy (LIBS) and X-ray fluorescence (XRF) spectroscopy, respectively. For LIBS, a significant decrease of Ca, P, and Na was observed in the young coffee group relative to agematched controls, whereas a significant increase in Mn, Fe, and K was observed. In the old coffee group, a significant increase of Mg, Fe, and K was observed along with a decrease of Mg, Ca, P, Na, Sr and Zn. For XRF, a significant decrease of the Ca/P ratio in the coffee group was observed. The SEM analysis showed pores and open spaces between young and old coffee groups, respectively. Thinning of enamel layers, loss of continuity in the enamel-dentin-junction, and wide spaces in dentin tubules with coffee use was found histologically. Coffee induces decalcification of teeth that corresponds to erosion, exposing the dentin structure by reducing enamel. Coffee immersion demonstrated an intrinsic staining in dentin by metal deposition.

Research Area(s)

  • Coffee, Erosion, Laser, LIBS, Teeth, X-ray, XRF

Bibliographic Note

Full text of this publication does not contain sufficient affiliation information. With consent from the author(s) concerned, the Research Unit(s) information for this record is based on the existing academic department affiliation of the author(s).

Citation Format(s)

Spectroscopic examination of enamel and dentin indicates erosion and staining by coffee through sequestration of elements. / Manno, Sinai H. C.; Manno, Francis A. M.; Lau, Condon.

Proceedings of SPIE: Lasers in Dentistry XXV. ed. / Peter Rechmann; Daniel Fried. Vol. 10857 SPIE, 2019. 108570K (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10857).

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review