Specificity of the ferrous oxidation of xylenol orange assay: Analysis of autoxidation products of cholesteryl arachidonate

Huiyong Yin; Ned A. Porter

doi:10.1016/S0003-2697(02)00621-8

Specificity of the ferrous oxidation of xylenol orange assay: Analysis of autoxidation products of cholesteryl arachidonate

Huiyong Yin, Ned A. Porter

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

42 Citations (Scopus)

Abstract

Autoxidation of polyunsaturated fatty acids and esters leads to a complex mixture containing hydroperoxides and cyclic peroxides. The oxidation mixture of cholesteryl arachidonate, which has been characterized by a variety of mass spectrometry techniques, was subject to analysis by conventional thiobarbituric acid-reactive substance (TBARS) and ferrous oxidation in xylenol orange (FOX) assays. Our results indicate that the FOX assay is not specific for hydroperoxides. Cyclic peroxides, such as monocyclic peroxides and serial-cyclic peroxides, give a positive FOX response even after triphenylphosphine reduction. We suggest that bicyclic endoperoxides are the major TBARS active compounds present in cholesteryl arachidonate oxidation mixtures. These compounds give a positive FOX assay before reaction with triphenylphosphine but negative TBARS and FOX assays after this reaction. Caution should be exercised when the FOX assay is used to analyze highly oxidized lipids, especially arachidonyl-containing lipids. © 2003 Elsevier Science (USA). All rights reserved.

Original language	English
Pages (from-to)	319-326
Journal	Analytical Biochemistry
Volume	313
Issue number	2
DOIs	https://doi.org/10.1016/S0003-2697(02)00621-8
Publication status	Published - 15 Feb 2003
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Research Keywords

Atherosclerosis
Cholesteryl arachidonate
Cyclic peroxides
FOX assay
Lipid hydroperoxides
Lipid peroxidation
Specificity
TBARS

Access Output

10.1016/S0003-2697(02)00621-8

Other Access Options

Check CityUHK Library

Permanent Link

Right click to copy link

Cite this

@article{90e18626be754b649eaa6a3aa7177425,

title = "Specificity of the ferrous oxidation of xylenol orange assay: Analysis of autoxidation products of cholesteryl arachidonate",

abstract = "Autoxidation of polyunsaturated fatty acids and esters leads to a complex mixture containing hydroperoxides and cyclic peroxides. The oxidation mixture of cholesteryl arachidonate, which has been characterized by a variety of mass spectrometry techniques, was subject to analysis by conventional thiobarbituric acid-reactive substance (TBARS) and ferrous oxidation in xylenol orange (FOX) assays. Our results indicate that the FOX assay is not specific for hydroperoxides. Cyclic peroxides, such as monocyclic peroxides and serial-cyclic peroxides, give a positive FOX response even after triphenylphosphine reduction. We suggest that bicyclic endoperoxides are the major TBARS active compounds present in cholesteryl arachidonate oxidation mixtures. These compounds give a positive FOX assay before reaction with triphenylphosphine but negative TBARS and FOX assays after this reaction. Caution should be exercised when the FOX assay is used to analyze highly oxidized lipids, especially arachidonyl-containing lipids. {\textcopyright} 2003 Elsevier Science (USA). All rights reserved.",

keywords = "Atherosclerosis, Cholesteryl arachidonate, Cyclic peroxides, FOX assay, Lipid hydroperoxides, Lipid peroxidation, Specificity, TBARS",

author = "Huiyong Yin and Porter, {Ned A.}",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].",

year = "2003",

month = feb,

day = "15",

doi = "10.1016/S0003-2697(02)00621-8",

language = "English",

volume = "313",

pages = "319--326",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Specificity of the ferrous oxidation of xylenol orange assay

T2 - Analysis of autoxidation products of cholesteryl arachidonate

AU - Yin, Huiyong

AU - Porter, Ned A.

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2003/2/15

Y1 - 2003/2/15

N2 - Autoxidation of polyunsaturated fatty acids and esters leads to a complex mixture containing hydroperoxides and cyclic peroxides. The oxidation mixture of cholesteryl arachidonate, which has been characterized by a variety of mass spectrometry techniques, was subject to analysis by conventional thiobarbituric acid-reactive substance (TBARS) and ferrous oxidation in xylenol orange (FOX) assays. Our results indicate that the FOX assay is not specific for hydroperoxides. Cyclic peroxides, such as monocyclic peroxides and serial-cyclic peroxides, give a positive FOX response even after triphenylphosphine reduction. We suggest that bicyclic endoperoxides are the major TBARS active compounds present in cholesteryl arachidonate oxidation mixtures. These compounds give a positive FOX assay before reaction with triphenylphosphine but negative TBARS and FOX assays after this reaction. Caution should be exercised when the FOX assay is used to analyze highly oxidized lipids, especially arachidonyl-containing lipids. © 2003 Elsevier Science (USA). All rights reserved.

AB - Autoxidation of polyunsaturated fatty acids and esters leads to a complex mixture containing hydroperoxides and cyclic peroxides. The oxidation mixture of cholesteryl arachidonate, which has been characterized by a variety of mass spectrometry techniques, was subject to analysis by conventional thiobarbituric acid-reactive substance (TBARS) and ferrous oxidation in xylenol orange (FOX) assays. Our results indicate that the FOX assay is not specific for hydroperoxides. Cyclic peroxides, such as monocyclic peroxides and serial-cyclic peroxides, give a positive FOX response even after triphenylphosphine reduction. We suggest that bicyclic endoperoxides are the major TBARS active compounds present in cholesteryl arachidonate oxidation mixtures. These compounds give a positive FOX assay before reaction with triphenylphosphine but negative TBARS and FOX assays after this reaction. Caution should be exercised when the FOX assay is used to analyze highly oxidized lipids, especially arachidonyl-containing lipids. © 2003 Elsevier Science (USA). All rights reserved.

KW - Atherosclerosis

KW - Cholesteryl arachidonate

KW - Cyclic peroxides

KW - FOX assay

KW - Lipid hydroperoxides

KW - Lipid peroxidation

KW - Specificity

KW - TBARS

UR - http://www.scopus.com/inward/record.url?scp=0037442449&partnerID=8YFLogxK

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-0037442449&origin=recordpage

U2 - 10.1016/S0003-2697(02)00621-8

DO - 10.1016/S0003-2697(02)00621-8

M3 - RGC 21 - Publication in refereed journal

C2 - 12605870

SN - 0003-2697

VL - 313

SP - 319

EP - 326

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Specificity of the ferrous oxidation of xylenol orange assay: Analysis of autoxidation products of cholesteryl arachidonate

Abstract

Bibliographical note

Research Keywords

Access Output

Other Access Options

Permanent Link

Other Files and Links

Fingerprint

Cite this