Specific down-regulation of spermatogenesis genes targeted by 22G RNAs in hybrid sterile males associated with an X-Chromosome introgression

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

19 Scopus Citations
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Author(s)

  • Xiaoliang Ren
  • Yu Bi
  • Vincy Wing Sze Ho
  • Chia-Ling Hsieh
  • Amanda Young
  • Zhihong Zhang
  • Tingting Lin
  • Yanmei Zhao
  • Long Miao
  • Peter Sarkies
  • Zhongying Zhao

Detail(s)

Original languageEnglish
Pages (from-to)1219-1232
Number of pages14
Journal / PublicationGenome Research
Volume26
Issue number9
Online published18 May 2016
Publication statusPublished - Sept 2016
Externally publishedYes

Link(s)

Abstract

Hybrid incompatibility (HI) prevents gene flow between species, thus lying at the heart of speciation genetics. One of the most common HIs is male sterility. Two superficially contradictory observations exist for hybrid male sterility. First, an introgression on the X Chromosome is more likely to produce male sterility than on autosome (so-called large-X theory); second, spermatogenesis genes are enriched on the autosomes but depleted on the X Chromosome (demasculinization of X Chromosome). Analysis of gene expression in Drosophila hybrids suggests a genetic interaction between the X Chromosome and autosomes that is essential for male fertility. However, the prevalence of such an interaction and its underlying mechanism remain largely unknown. Here we examine the interaction in nematode species by contrasting the expression of both coding genes and transposable elements (TEs) between hybrid sterile males and its parental nematode males. We use two lines of hybrid sterile males, each carrying an independent introgression fragment from Caenorhabditis briggsae X Chromosome in an otherwise Caenorhabditis nigoni background, which demonstrate similar defects in spermatogenesis. We observe a similar pattern of down-regulated genes that are specific for spermatogenesis between the two hybrids. Importantly, the down-regulated genes caused by the X Chromosome introgressions show a significant enrichment on the autosomes, supporting an epistatic interaction between the X Chromosome and autosomes. We investigate the underlying mechanism of the interaction by measuring small RNAs and find that a subset of 22G RNAs specifically targeting the down-regulated spermatogenesis genes is significantly up-regulated in hybrids, suggesting that perturbation of small RNA-mediated regulation may contribute to the X-autosome interaction.

Research Area(s)

  • Animals, Caenorhabditis/genetics, Gene Expression Regulation, Developmental, Gene Flow, Genetic Speciation, Hybridization, Genetic, Infertility, Male/genetics, Male, RNA/genetics, Spermatogenesis/genetics, X Chromosome/genetics

Bibliographic Note

© 2016 Li et al.; Published by Cold Spring Harbor Laboratory Press.

Citation Format(s)

Specific down-regulation of spermatogenesis genes targeted by 22G RNAs in hybrid sterile males associated with an X-Chromosome introgression. / Li, Runsheng; Ren, Xiaoliang; Bi, Yu et al.
In: Genome Research, Vol. 26, No. 9, 09.2016, p. 1219-1232.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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