Specific Binding of a D-RNA G-Quadruplex Structure with an L-RNA Aptamer

Chun-Yin Chan; Chun Kit Kwok

doi:10.1002/anie.201914955

Specific Binding of a D-RNA G-Quadruplex Structure with an L-RNA Aptamer

Chun-Yin Chan, Chun Kit Kwok^*

^*Corresponding author for this work

Department of Chemistry

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

42 Citations (Scopus)

Abstract

G-quadruplex (G4) structures are of general importance in chemistry and biology, such as in biosensing, gene regulation, and cancers. Although a large repertoire of G4-binding tools has been developed, no aptamer has been developed to interact with G4. Moreover, the G4 selectivity of current toolkits is very limited. Herein, we report the first L-RNA aptamer that targets a D-RNA G-quadruplex (rG4). Using TERRA rG4 as an example, our results reveal that this l-RNA aptamer, Ap3-7, folds into a unique secondary structure, exhibits high G4 selectivity and effectively interferes with TERRA-rG4–RHAU53 binding. Our approach and findings open a new door in further developing G4-specific tools for diverse applications.

Original language	English
Pages (from-to)	5293-5297
Journal	Angewandte Chemie - International Edition
Volume	59
Issue number	13
Online published	23 Jan 2020
DOIs	https://doi.org/10.1002/anie.201914955
Publication status	Published - 23 Mar 2020

Research Keywords

aptamers
G-quadruplexes
nucleic acids
RNA
RNA structures

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access Output

10.1002/anie.201914955

Other Access Options

Check CityUHK Library

Permanent Link

Right click to copy link

Cite this

@article{5c093dd70a33407f8ade42deeb3837e5,

title = "Specific Binding of a D-RNA G-Quadruplex Structure with an L-RNA Aptamer",

abstract = "G-quadruplex (G4) structures are of general importance in chemistry and biology, such as in biosensing, gene regulation, and cancers. Although a large repertoire of G4-binding tools has been developed, no aptamer has been developed to interact with G4. Moreover, the G4 selectivity of current toolkits is very limited. Herein, we report the first L-RNA aptamer that targets a D-RNA G-quadruplex (rG4). Using TERRA rG4 as an example, our results reveal that this l-RNA aptamer, Ap3-7, folds into a unique secondary structure, exhibits high G4 selectivity and effectively interferes with TERRA-rG4–RHAU53 binding. Our approach and findings open a new door in further developing G4-specific tools for diverse applications.",

keywords = "aptamers, G-quadruplexes, nucleic acids, RNA, RNA structures",

author = "Chun-Yin Chan and Kwok, {Chun Kit}",

year = "2020",

month = mar,

day = "23",

doi = "10.1002/anie.201914955",

language = "English",

volume = "59",

pages = "5293--5297",

journal = "Angewandte Chemie - International Edition",

issn = "1433-7851",

publisher = "John Wiley & Sons",

number = "13",

}

TY - JOUR

T1 - Specific Binding of a D-RNA G-Quadruplex Structure with an L-RNA Aptamer

AU - Chan, Chun-Yin

AU - Kwok, Chun Kit

PY - 2020/3/23

Y1 - 2020/3/23

N2 - G-quadruplex (G4) structures are of general importance in chemistry and biology, such as in biosensing, gene regulation, and cancers. Although a large repertoire of G4-binding tools has been developed, no aptamer has been developed to interact with G4. Moreover, the G4 selectivity of current toolkits is very limited. Herein, we report the first L-RNA aptamer that targets a D-RNA G-quadruplex (rG4). Using TERRA rG4 as an example, our results reveal that this l-RNA aptamer, Ap3-7, folds into a unique secondary structure, exhibits high G4 selectivity and effectively interferes with TERRA-rG4–RHAU53 binding. Our approach and findings open a new door in further developing G4-specific tools for diverse applications.

AB - G-quadruplex (G4) structures are of general importance in chemistry and biology, such as in biosensing, gene regulation, and cancers. Although a large repertoire of G4-binding tools has been developed, no aptamer has been developed to interact with G4. Moreover, the G4 selectivity of current toolkits is very limited. Herein, we report the first L-RNA aptamer that targets a D-RNA G-quadruplex (rG4). Using TERRA rG4 as an example, our results reveal that this l-RNA aptamer, Ap3-7, folds into a unique secondary structure, exhibits high G4 selectivity and effectively interferes with TERRA-rG4–RHAU53 binding. Our approach and findings open a new door in further developing G4-specific tools for diverse applications.

KW - aptamers

KW - G-quadruplexes

KW - nucleic acids

KW - RNA

KW - RNA structures

UR - http://www.scopus.com/inward/record.url?scp=85079452606&partnerID=8YFLogxK

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85079452606&origin=recordpage

U2 - 10.1002/anie.201914955

DO - 10.1002/anie.201914955

M3 - RGC 21 - Publication in refereed journal

SN - 1433-7851

VL - 59

SP - 5293

EP - 5297

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 13

ER -

Specific Binding of a D-RNA G-Quadruplex Structure with an L-RNA Aptamer

Abstract

Research Keywords

UN SDGs

Access Output

Other Access Options

Permanent Link

Other Files and Links

Fingerprint

CROU: Deciphering Long Non-coding RNA Structures, Interactions, and their Functions in Skeletal Myogenic Differentiation

GRF: Mapping and Targeting of RNA G-quadruplex Structures in the Human Non-coding Transcriptome

GRF: Interrogating the Effect of 3'UTR RNA G-quadruplex Structure in MicroRNA Target Site Accessibility and Translational Regulation

Cite this

Specific Binding of a D-RNA G-Quadruplex Structure with an L-RNA Aptamer

Abstract

Research Keywords

UN SDGs

Access Output

Other Access Options

Permanent Link

Other Files and Links

Fingerprint

Projects

CROU: Deciphering Long Non-coding RNA Structures, Interactions, and their Functions in Skeletal Myogenic Differentiation

GRF: Mapping and Targeting of RNA G-quadruplex Structures in the Human Non-coding Transcriptome

GRF: Interrogating the Effect of 3'UTR RNA G-quadruplex Structure in MicroRNA Target Site Accessibility and Translational Regulation

Cite this