Smart Hydrogel Microfluidics for Single-Cell Multiplexed Secretomic Analysis with High Sensitivity

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

15 Scopus Citations
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Author(s)

  • Myat Noe Hsu
  • Shih-Chung Wei
  • Song Guo
  • Dinh-Tuan Phan
  • Yong Zhang

Detail(s)

Original languageEnglish
Article number1802918
Journal / PublicationSmall
Volume14
Issue number49
Online published17 Oct 2018
Publication statusPublished - 6 Dec 2018
Externally publishedYes

Abstract

Secreted proteins determine a range of cellular functionalities correlated with human health and disease progression. Because of cell heterogeneity, it is essential to measure low abundant protein secretions from individual cells to determine single-cell activities. In this study, an integrated platform consisting of smart hydrogel immunosensors for the sensitive detection of single-cell secretions is developed. A single cell and smart hydrogel microparticles are encapsulated within a droplet. After incubation, target secreted proteins from the cell are captured in the smart hydrogel particle for immunoassay. The temperature-induced volume phase transition of the hydrogel biosensor allows the concentration of analytes within the gel matrix to increase, enabling high-sensitivity measurements. Distinct heterogeneity for live cell secretions is determined from 6000 cells within 1 h. This method is tested for low abundant essential secretions, such as interleukin-6, interleukin-8, and monocyte chemoattractant protein-1 secretions of both suspended cells (HL60) and adherent cells (MCF7 and MDA-MB-231). This platform is highly flexible and can be used to simultaneously measure a wide range of clinically relevant cellular secretions; it thus represents a novel tool for precise biological assays.

Research Area(s)

  • droplet microfluidics, hydrogel microparticles, immunofluorescence assays, signal enhancements, single cell protein secretions

Citation Format(s)

Smart Hydrogel Microfluidics for Single-Cell Multiplexed Secretomic Analysis with High Sensitivity. / Hsu, Myat Noe; Wei, Shih-Chung; Guo, Song; Phan, Dinh-Tuan; Zhang, Yong; Chen, Chia-Hung.

In: Small, Vol. 14, No. 49, 1802918, 06.12.2018.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal