Skin-interfaced microfluidic biosensors for colorimetric measurements of the concentrations of ketones in sweat

Yunyun Wu; Xinming Li; Kenneth E. Madsen; Haohui Zhang; Soongwon Cho; Ruihao Song; Ravi F. Nuxoll; Yirui Xiong; Jiaqi Liu; Jingyuan Feng; Tianyu Yang; Kaiqing Zhang; Alexander J. Aranyosi; Donald E. Wright; Roozbeh Ghaffari; Yonggang Huang; Ralph G. Nuzzo; John A. Rogers

doi:10.1039/d4lc00588k

Skin-interfaced microfluidic biosensors for colorimetric measurements of the concentrations of ketones in sweat

Yunyun Wu, Xinming Li, Kenneth E. Madsen, Haohui Zhang, Soongwon Cho, Ruihao Song, Ravi F. Nuxoll, Yirui Xiong, Jiaqi Liu, Jingyuan Feng, Tianyu Yang, Kaiqing Zhang, Alexander J. Aranyosi, Donald E. Wright, Roozbeh Ghaffari, Yonggang Huang, Ralph G. Nuzzo^*, John A. Rogers^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

10 Citations (Scopus)

Abstract

Ketones, such as beta-hydroxybutyrate (BHB), are important metabolites that can be used to monitor for conditions such as diabetic ketoacidosis (DKA) and ketosis. Compared to conventional approaches that rely on samples of urine or blood evaluated using laboratory techniques, processes for monitoring of ketones in sweat using on-body sensors offer significant advantages. Here, we report a class of soft, skin-interfaced microfluidic devices that can quantify the concentrations of BHB in sweat based on simple and low-cost colorimetric schemes. These devices combine microfluidic structures and enzymatic colorimetric BHB assays for selective and accurate analysis. Human trials demonstrate the broad applicability of this technology in practical scenarios, and they also establish quantitative correlations between the concentration of BHB in sweat and in blood. The results represent a convenient means for managing DKA and aspects of personal nutrition/wellness. © 2024 The Royal Society of Chemistry.

Original language	English
Pages (from-to)	4288-4295
Number of pages	8
Journal	Lab on a Chip
Volume	24
Issue number	18
Online published	28 Aug 2024
DOIs	https://doi.org/10.1039/d4lc00588k
Publication status	Published - 21 Sept 2024
Externally published	Yes

Funding

This work was supported by the Center for Bio-Integrated Electronics of Querrey Simpson Institute for Bioelectronics at Northwestern University. This work made use of the EPIC and IMSERC facility of Northwestern University's NUANCE Center, which has received support from the SHyNE Resource (NSF ECCS-2025633), the IIN, and Northwestern's MRSEC program (NSF DMR-1720139).

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This full text is made available under CC-BY 3.0. https://creativecommons.org/licenses/by/3.0/

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Wu, Y., Li, X., Madsen, K. E., Zhang, H., Cho, S., Song, R., Nuxoll, R. F., Xiong, Y., Liu, J., Feng, J., Yang, T., Zhang, K., Aranyosi, A. J., Wright, D. E., Ghaffari, R., Huang, Y., Nuzzo, R. G., & Rogers, J. A. (2024). Skin-interfaced microfluidic biosensors for colorimetric measurements of the concentrations of ketones in sweat. Lab on a Chip, 24(18), 4288-4295. https://doi.org/10.1039/d4lc00588k

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title = "Skin-interfaced microfluidic biosensors for colorimetric measurements of the concentrations of ketones in sweat",

abstract = "Ketones, such as beta-hydroxybutyrate (BHB), are important metabolites that can be used to monitor for conditions such as diabetic ketoacidosis (DKA) and ketosis. Compared to conventional approaches that rely on samples of urine or blood evaluated using laboratory techniques, processes for monitoring of ketones in sweat using on-body sensors offer significant advantages. Here, we report a class of soft, skin-interfaced microfluidic devices that can quantify the concentrations of BHB in sweat based on simple and low-cost colorimetric schemes. These devices combine microfluidic structures and enzymatic colorimetric BHB assays for selective and accurate analysis. Human trials demonstrate the broad applicability of this technology in practical scenarios, and they also establish quantitative correlations between the concentration of BHB in sweat and in blood. The results represent a convenient means for managing DKA and aspects of personal nutrition/wellness. {\textcopyright} 2024 The Royal Society of Chemistry.",

author = "Yunyun Wu and Xinming Li and Madsen, {Kenneth E.} and Haohui Zhang and Soongwon Cho and Ruihao Song and Nuxoll, {Ravi F.} and Yirui Xiong and Jiaqi Liu and Jingyuan Feng and Tianyu Yang and Kaiqing Zhang and Aranyosi, {Alexander J.} and Wright, {Donald E.} and Roozbeh Ghaffari and Yonggang Huang and Nuzzo, {Ralph G.} and Rogers, {John A.}",

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Wu, Y, Li, X, Madsen, KE, Zhang, H, Cho, S, Song, R, Nuxoll, RF, Xiong, Y, Liu, J, Feng, J, Yang, T, Zhang, K, Aranyosi, AJ, Wright, DE, Ghaffari, R, Huang, Y, Nuzzo, RG & Rogers, JA 2024, 'Skin-interfaced microfluidic biosensors for colorimetric measurements of the concentrations of ketones in sweat', Lab on a Chip, vol. 24, no. 18, pp. 4288-4295. https://doi.org/10.1039/d4lc00588k

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T1 - Skin-interfaced microfluidic biosensors for colorimetric measurements of the concentrations of ketones in sweat

AU - Wu, Yunyun

AU - Li, Xinming

AU - Madsen, Kenneth E.

AU - Zhang, Haohui

AU - Cho, Soongwon

AU - Song, Ruihao

AU - Nuxoll, Ravi F.

AU - Xiong, Yirui

AU - Liu, Jiaqi

AU - Feng, Jingyuan

AU - Yang, Tianyu

AU - Zhang, Kaiqing

AU - Aranyosi, Alexander J.

AU - Wright, Donald E.

AU - Ghaffari, Roozbeh

AU - Huang, Yonggang

AU - Nuzzo, Ralph G.

AU - Rogers, John A.

PY - 2024/9/21

Y1 - 2024/9/21

N2 - Ketones, such as beta-hydroxybutyrate (BHB), are important metabolites that can be used to monitor for conditions such as diabetic ketoacidosis (DKA) and ketosis. Compared to conventional approaches that rely on samples of urine or blood evaluated using laboratory techniques, processes for monitoring of ketones in sweat using on-body sensors offer significant advantages. Here, we report a class of soft, skin-interfaced microfluidic devices that can quantify the concentrations of BHB in sweat based on simple and low-cost colorimetric schemes. These devices combine microfluidic structures and enzymatic colorimetric BHB assays for selective and accurate analysis. Human trials demonstrate the broad applicability of this technology in practical scenarios, and they also establish quantitative correlations between the concentration of BHB in sweat and in blood. The results represent a convenient means for managing DKA and aspects of personal nutrition/wellness. © 2024 The Royal Society of Chemistry.

AB - Ketones, such as beta-hydroxybutyrate (BHB), are important metabolites that can be used to monitor for conditions such as diabetic ketoacidosis (DKA) and ketosis. Compared to conventional approaches that rely on samples of urine or blood evaluated using laboratory techniques, processes for monitoring of ketones in sweat using on-body sensors offer significant advantages. Here, we report a class of soft, skin-interfaced microfluidic devices that can quantify the concentrations of BHB in sweat based on simple and low-cost colorimetric schemes. These devices combine microfluidic structures and enzymatic colorimetric BHB assays for selective and accurate analysis. Human trials demonstrate the broad applicability of this technology in practical scenarios, and they also establish quantitative correlations between the concentration of BHB in sweat and in blood. The results represent a convenient means for managing DKA and aspects of personal nutrition/wellness. © 2024 The Royal Society of Chemistry.

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JO - Lab on a Chip

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Skin-interfaced microfluidic biosensors for colorimetric measurements of the concentrations of ketones in sweat

Abstract

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