Single-cell profiling approaches to probing tumor heterogeneity

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

45 Scopus Citations
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Author(s)

  • Parthiv Kant Chaudhuri
  • Naveen Ramalingam
  • Daniel Shao Weng Tan
  • Chwee Teck Lim
  • Majid Ebrahimi Warkiani

Detail(s)

Original languageEnglish
Pages (from-to)243-255
Journal / PublicationInternational Journal of Cancer
Volume139
Issue number2
Online published20 Jan 2016
Publication statusPublished - 15 Jul 2016
Externally publishedYes

Abstract

Tumor heterogeneity is a major hindrance in cancer classification, diagnosis and treatment. Recent technological advances have begun to reveal the true extent of its heterogeneity. Single-cell analysis (SCA) is emerging as an important approach to detect variations in morphology, genetic or proteomic expression. In this review, we revisit the issue of inter- and intra-tumor heterogeneity, and list various modes of SCA techniques (cell-based, nucleic acid-based, protein-based, metabolite-based and lipid-based) presently used for cancer characterization. We further discuss the advantages of SCA over pooled cell analysis, as well as the limitations of conventional techniques. Emerging trends, such as high-throughput sequencing, are also mentioned as improved means for cancer profiling. Collectively, these applications have the potential for breakthroughs in cancer treatment.

Research Area(s)

  • cancer, heterogeneity, microfluidics, molecular analysis, single-cell analysis, tumors

Citation Format(s)

Single-cell profiling approaches to probing tumor heterogeneity. / Khoo, Bee Luan; Chaudhuri, Parthiv Kant; Ramalingam, Naveen et al.

In: International Journal of Cancer, Vol. 139, No. 2, 15.07.2016, p. 243-255.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review