Single-cell encoded gene silencing for high-throughput combinatorial siRNA screening
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Author(s)
Related Research Unit(s)
Detail(s)
Original language | English |
---|---|
Article number | 9985 |
Journal / Publication | Nature Communications |
Volume | 15 |
Online published | 19 Nov 2024 |
Publication status | Published - 2024 |
Link(s)
DOI | DOI |
---|---|
Attachment(s) | Documents
Publisher's Copyright Statement
|
Link to Scopus | https://www.scopus.com/record/display.uri?eid=2-s2.0-85209398766&origin=recordpage |
Permanent Link | https://scholars.cityu.edu.hk/en/publications/publication(6de426be-1dc6-4a22-8eef-7cc93946bde8).html |
Abstract
The use of combinatorial siRNAs shows great promise for drug discovery, but the identification of safe and effective siRNA combinations remains challenging. Here, we develop a massively multiplexed technology for systematic screening of siRNA-based cocktail therapeutics. We employ composite micro-carriers that are responsive to near infrared light and magnetic field to achieve photoporation-facilitated siRNA transfection to individual cells. Thus, randomized gene silencing by different siRNA formulations can be performed with high-throughput single-cell-based analyses. For screening anti-cancer siRNA cocktails, we test more than 1300 siRNA combinations for knocking down multiple genes related to tumor growth, discovering effective 3-siRNA formulations with an emphasis on the critical role of inhibiting Cyclin D1 and survivin, along with their complementary targets for synergic efficacy. This approach enables orders of magnitude reduction in time and cost associated with largescale siRNA screening, and resolves key insights to siRNA pharmacology that are not permissive to existing methods. © The Author(s) 2024.
Research Area(s)
Citation Format(s)
Single-cell encoded gene silencing for high-throughput combinatorial siRNA screening. / Guo, Feng; Ji, Xianglin; Xiong, Chuxiao et al.
In: Nature Communications, Vol. 15, 9985, 2024.
In: Nature Communications, Vol. 15, 9985, 2024.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Download Statistics
No data available