Abstract
We present fast functional optical-resolution photoacoustic microscopy (OR-PAM) that can simultaneously image hemoglobin concentration, blood flow speed, and oxygen saturation with three-pulse excitation. To instantaneously determine the blood flow speed, dual-pulse photoacoustic flowmetry is developed to determine the blood flow speed from photoacoustic signal decay in sub-microseconds. Grueneisen relaxation effect is compensated for in the oxygen saturation calculation. The blood flow imaging is validated in phantom and in vivo experiments. The results show that the flow speed can be measured accurately in sub-microseconds by comparing the dual-pulse flowmetric method with photoacoustic Doppler flowmetry. Wide-field OR-PAM of hemoglobin concentration, blood flow speed, and oxygen saturation are demonstrated in the mouse ear. This technical advance enables more biomedical applications for fast functional OR-PAM.
| Original language | English |
|---|---|
| Article number | 100156 |
| Journal | Photoacoustics |
| Volume | 17 |
| Online published | 26 Dec 2019 |
| DOIs | |
| Publication status | Published - Mar 2020 |
Research Keywords
- Dual-pulse photoacoustic flowmetry
- Grueneisen relaxation effect
- Multi-functional
- Sub-microseconds
Publisher's Copyright Statement
- This full text is made available under CC-BY-NC-ND 4.0. https://creativecommons.org/licenses/by-nc-nd/4.0/
RGC Funding Information
- RGC-funded
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Dive into the research topics of 'Single-shot photoacoustic microscopy of hemoglobin concentration, oxygen saturation, and blood flow in sub-microseconds'. Together they form a unique fingerprint.Projects
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ECS: Large-Scale Single-Cell Photoacoustic Microscopy of the Brain in Action
WANG, L. (Principal Investigator / Project Coordinator)
1/01/17 → 30/12/20
Project: Research
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