Single-cell Sequencing of Circulating Tumor Cells: Recent Technical Advances, Challenges and Applications

Ying NI; Yuzhu CUI; Heng ZOU; Tao XU; Mengsu YANG

doi:10.1039/9781788019958-00169

Single-cell Sequencing of Circulating Tumor Cells: Recent Technical Advances, Challenges and Applications

Ying NI
, Yuzhu CUI
, Heng ZOU
, Tao XU^*
, Mengsu YANG^*

^*Corresponding author for this work

Department of Biomedical Sciences

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 12 - Chapter in an edited book (Author) › peer-review

Abstract

Single-cell sequencing has provided precision medicine with a new perspective for cancer detection because of its power to reveal cellular heterogeneity, especially in tumorigenesis. The application of next-generation sequencing (NGS) technique in circulating tumor cells (CTCs) further enhances the capability of cancer diagnosis and monitoring through liquid biopsy. Emerging technological developments in CTC enrichment and isolation, whole-genome amplification, single-cell sequencing analysis, and their applications on translational research and clinic practice are summarized in this chapter. The review also discusses the technical challenges in transforming CTC information and single CTC sequencing platform from academic research to practical use. Critical research questions are also highlighted in order to help provide clinical benefits for cancer patients using CTC-based single-cell sequencing techniques. © The Royal Society of Chemistry 2021.

Original language	English
Title of host publication	Detection Methods in Precision Medicine
Editors	Mengsu Yang, Michael Thompson
Publisher	Royal Society of Chemistry
Chapter	9
Pages	169-190
ISBN (Electronic)	978-1-78801-996-5
ISBN (Print)	978-1-78801-761-9, 978-1-78801-995-8
DOIs	https://doi.org/10.1039/9781788019958-00169
Publication status	Published - 2021

Publication series

Name	Detection Science Series
Number	18
ISSN (Print)	2052-3068
ISSN (Electronic)	2052-3076

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Keywords

WHOLE-GENOME AMPLIFICATION
MESSENGER-RNA-SEQ
CLINICAL-APPLICATIONS
CANCER-PATIENTS
DNA
HETEROGENEITY
METASTASIS
NUCLEOTIDE
LANDSCAPE
EVOLUTION

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abstract = "Single-cell sequencing has provided precision medicine with a new perspective for cancer detection because of its power to reveal cellular heterogeneity, especially in tumorigenesis. The application of next-generation sequencing (NGS) technique in circulating tumor cells (CTCs) further enhances the capability of cancer diagnosis and monitoring through liquid biopsy. Emerging technological developments in CTC enrichment and isolation, whole-genome amplification, single-cell sequencing analysis, and their applications on translational research and clinic practice are summarized in this chapter. The review also discusses the technical challenges in transforming CTC information and single CTC sequencing platform from academic research to practical use. Critical research questions are also highlighted in order to help provide clinical benefits for cancer patients using CTC-based single-cell sequencing techniques. {\textcopyright} The Royal Society of Chemistry 2021.",

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author = "Ying NI and Yuzhu CUI and Heng ZOU and Tao XU and Mengsu YANG",

year = "2021",

doi = "10.1039/9781788019958-00169",

language = "English",

isbn = "978-1-78801-761-9",

series = "Detection Science Series",

publisher = "Royal Society of Chemistry",

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pages = "169--190",

editor = "Mengsu Yang and Michael Thompson",

booktitle = "Detection Methods in Precision Medicine",

address = "United Kingdom",

}

Single-cell Sequencing of Circulating Tumor Cells: Recent Technical Advances, Challenges and Applications. / NI, Ying; CUI, Yuzhu; ZOU, Heng et al.
Detection Methods in Precision Medicine. ed. / Mengsu Yang; Michael Thompson. Royal Society of Chemistry, 2021. p. 169-190 (Detection Science Series; No. 18).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 12 - Chapter in an edited book (Author) › peer-review

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AU - CUI, Yuzhu

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AU - YANG, Mengsu

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N2 - Single-cell sequencing has provided precision medicine with a new perspective for cancer detection because of its power to reveal cellular heterogeneity, especially in tumorigenesis. The application of next-generation sequencing (NGS) technique in circulating tumor cells (CTCs) further enhances the capability of cancer diagnosis and monitoring through liquid biopsy. Emerging technological developments in CTC enrichment and isolation, whole-genome amplification, single-cell sequencing analysis, and their applications on translational research and clinic practice are summarized in this chapter. The review also discusses the technical challenges in transforming CTC information and single CTC sequencing platform from academic research to practical use. Critical research questions are also highlighted in order to help provide clinical benefits for cancer patients using CTC-based single-cell sequencing techniques. © The Royal Society of Chemistry 2021.

AB - Single-cell sequencing has provided precision medicine with a new perspective for cancer detection because of its power to reveal cellular heterogeneity, especially in tumorigenesis. The application of next-generation sequencing (NGS) technique in circulating tumor cells (CTCs) further enhances the capability of cancer diagnosis and monitoring through liquid biopsy. Emerging technological developments in CTC enrichment and isolation, whole-genome amplification, single-cell sequencing analysis, and their applications on translational research and clinic practice are summarized in this chapter. The review also discusses the technical challenges in transforming CTC information and single CTC sequencing platform from academic research to practical use. Critical research questions are also highlighted in order to help provide clinical benefits for cancer patients using CTC-based single-cell sequencing techniques. © The Royal Society of Chemistry 2021.

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KW - CLINICAL-APPLICATIONS

KW - CANCER-PATIENTS

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KW - HETEROGENEITY

KW - METASTASIS

KW - NUCLEOTIDE

KW - LANDSCAPE

KW - EVOLUTION

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SN - 978-1-78801-995-8

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PB - Royal Society of Chemistry

ER -

Single-cell Sequencing of Circulating Tumor Cells: Recent Technical Advances, Challenges and Applications

Abstract

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UN SDGs

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