Single-cell photoacoustic thermometry

Liang Gao, Lidai Wang, Chiye Li, Yan Liu, Haixin Ke, Chi Zhang, Lihong V. Wang

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

70 Citations (Scopus)

Abstract

A novel photoacoustic thermometric method is presented for simultaneously imaging cells and sensing their temperature. With three-seconds-per-frame imaging speed, a temperature resolution of 0.2°C was achieved in a photo-thermal cell heating experiment. Compared to other approaches, the photoacoustic thermometric method has the advantage of not requiring custom-developed temperature-sensitive biosensors. This feature should facilitate the conversion of single-cell thermometry into a routine lab tool and make it accessible to a much broader biological research community. © Society of Photo-Optical Instrumentation Engineers.
Original languageEnglish
Article number26003
JournalJournal of Biomedical Optics
Volume18
Issue number2
DOIs
Publication statusPublished - Feb 2013
Externally publishedYes

Research Keywords

  • cell imaging
  • Photoacoustic microscopy
  • Single-cell thermometry

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