Single cell penetration using nano-pipette by E-SEM nanorobotic manipulation system

Yajing Shen; Masahiro Nakajima; Mohd Ridzuan Ahmad; Toshio Fukuda; Seiji Kojima; Michio Homma

doi:10.1109/ICMA.2009.5245024

Single cell penetration using nano-pipette by E-SEM nanorobotic manipulation system

Yajing Shen^*
, Masahiro Nakajima
, Mohd Ridzuan Ahmad
, Toshio Fukuda
, Seiji Kojima
, Michio Homma

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

8 Citations (Scopus)

Abstract

We performed in situ penetration of single wildtype yeast cell (W303) using nano-pipette inside an environmental scanning electron microscope (E-SEM). An E-SEM nanorobotic manipulator has been constructed with three units and seven degrees of freedom (DOFs) in total. Micro pulling and FIB etching were used to control the tip size of nano-pipette accurately. Cells were fixed on the cooling stage in a perpendicular direction with the nano-pipette. Nano-pipette was assembled on the nanomanipulator to apply penetration force on a single cell. The E-SEM can provide the real-time observation with nanoscale resolution during the experiment. Materials of pipette, translational speed, and tip diameter were three important factors to the successful penetration of yeast cell. We discussed these factors and pointed out the suitable parameters for yeast penetration. ©2009 IEEE.

Original language	English
Title of host publication	2009 IEEE International Conference on Mechatronics and Automation, ICMA 2009
Pages	1849-1854
DOIs	https://doi.org/10.1109/ICMA.2009.5245024
Publication status	Published - 2009
Externally published	Yes
Event	2009 IEEE International Conference on Mechatronics and Automation, ICMA 2009 - Changchun, China Duration: 9 Aug 2009 → 12 Aug 2009 https://ieeexplore.ieee.org/xpl/conhome/5226790/proceeding

Conference

Conference	2009 IEEE International Conference on Mechatronics and Automation, ICMA 2009
Place	China
City	Changchun
Period	9/08/09 → 12/08/09
Internet address	https://ieeexplore.ieee.org/xpl/conhome/5226790/proceeding

Research Keywords

Environmental-SEM
Nano-pipette
Nanomanipulation
Single cell analysis
Yeast cells

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10.1109/ICMA.2009.5245024

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@inproceedings{ace5a896a288492aaa282cb761990306,

title = "Single cell penetration using nano-pipette by E-SEM nanorobotic manipulation system",

abstract = "We performed in situ penetration of single wildtype yeast cell (W303) using nano-pipette inside an environmental scanning electron microscope (E-SEM). An E-SEM nanorobotic manipulator has been constructed with three units and seven degrees of freedom (DOFs) in total. Micro pulling and FIB etching were used to control the tip size of nano-pipette accurately. Cells were fixed on the cooling stage in a perpendicular direction with the nano-pipette. Nano-pipette was assembled on the nanomanipulator to apply penetration force on a single cell. The E-SEM can provide the real-time observation with nanoscale resolution during the experiment. Materials of pipette, translational speed, and tip diameter were three important factors to the successful penetration of yeast cell. We discussed these factors and pointed out the suitable parameters for yeast penetration. {\textcopyright}2009 IEEE.",

keywords = "Environmental-SEM, Nano-pipette, Nanomanipulation, Single cell analysis, Yeast cells",

author = "Yajing Shen and Masahiro Nakajima and Ahmad, \{Mohd Ridzuan\} and Toshio Fukuda and Seiji Kojima and Michio Homma",

year = "2009",

doi = "10.1109/ICMA.2009.5245024",

language = "English",

isbn = "9781424426935",

pages = "1849--1854",

booktitle = "2009 IEEE International Conference on Mechatronics and Automation, ICMA 2009",

note = "2009 IEEE International Conference on Mechatronics and Automation, ICMA 2009 ; Conference date: 09-08-2009 Through 12-08-2009",

url = "https://ieeexplore.ieee.org/xpl/conhome/5226790/proceeding",

}

Shen, Y, Nakajima, M, Ahmad, MR, Fukuda, T, Kojima, S & Homma, M 2009, Single cell penetration using nano-pipette by E-SEM nanorobotic manipulation system. in 2009 IEEE International Conference on Mechatronics and Automation, ICMA 2009., 5245024, pp. 1849-1854, 2009 IEEE International Conference on Mechatronics and Automation, ICMA 2009, Changchun, China, 9/08/09. https://doi.org/10.1109/ICMA.2009.5245024

Single cell penetration using nano-pipette by E-SEM nanorobotic manipulation system. / Shen, Yajing; Nakajima, Masahiro; Ahmad, Mohd Ridzuan et al.
2009 IEEE International Conference on Mechatronics and Automation, ICMA 2009. 2009. p. 1849-1854 5245024.

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - Single cell penetration using nano-pipette by E-SEM nanorobotic manipulation system

AU - Shen, Yajing

AU - Nakajima, Masahiro

AU - Ahmad, Mohd Ridzuan

AU - Fukuda, Toshio

AU - Kojima, Seiji

AU - Homma, Michio

PY - 2009

Y1 - 2009

N2 - We performed in situ penetration of single wildtype yeast cell (W303) using nano-pipette inside an environmental scanning electron microscope (E-SEM). An E-SEM nanorobotic manipulator has been constructed with three units and seven degrees of freedom (DOFs) in total. Micro pulling and FIB etching were used to control the tip size of nano-pipette accurately. Cells were fixed on the cooling stage in a perpendicular direction with the nano-pipette. Nano-pipette was assembled on the nanomanipulator to apply penetration force on a single cell. The E-SEM can provide the real-time observation with nanoscale resolution during the experiment. Materials of pipette, translational speed, and tip diameter were three important factors to the successful penetration of yeast cell. We discussed these factors and pointed out the suitable parameters for yeast penetration. ©2009 IEEE.

AB - We performed in situ penetration of single wildtype yeast cell (W303) using nano-pipette inside an environmental scanning electron microscope (E-SEM). An E-SEM nanorobotic manipulator has been constructed with three units and seven degrees of freedom (DOFs) in total. Micro pulling and FIB etching were used to control the tip size of nano-pipette accurately. Cells were fixed on the cooling stage in a perpendicular direction with the nano-pipette. Nano-pipette was assembled on the nanomanipulator to apply penetration force on a single cell. The E-SEM can provide the real-time observation with nanoscale resolution during the experiment. Materials of pipette, translational speed, and tip diameter were three important factors to the successful penetration of yeast cell. We discussed these factors and pointed out the suitable parameters for yeast penetration. ©2009 IEEE.

KW - Environmental-SEM

KW - Nano-pipette

KW - Nanomanipulation

KW - Single cell analysis

KW - Yeast cells

UR - https://www.scopus.com/pages/publications/77449154393

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U2 - 10.1109/ICMA.2009.5245024

DO - 10.1109/ICMA.2009.5245024

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781424426935

SP - 1849

EP - 1854

BT - 2009 IEEE International Conference on Mechatronics and Automation, ICMA 2009

T2 - 2009 IEEE International Conference on Mechatronics and Automation, ICMA 2009

Y2 - 9 August 2009 through 12 August 2009

ER -

Single cell penetration using nano-pipette by E-SEM nanorobotic manipulation system

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