@inproceedings{edb8062b7ca94306a1d3c92b679a5226,
title = "Single cell injection using nano pipette via nanorobotic manipulation system inside E-SEM",
abstract = "In this work, a nano injection system for single cell with small size (diameter: -5 μm) is proposed. An environmental scanning electron microscope (E-SEM) nanorobotic manipulation system has been constructed with three units and seven degrees of freedom (DOFs) in total. It can provide a real-time observation with nanoscale resolution during the experiment. Micro pulling and FIB etching are used to control the tip size of nano pipette accurately. In situ penetration of single wild-type yeast cell (W303) is performed using nano pipette inside the E-SEM based on the nanorobotic manipulation system. Spouting experiment of Rhodamine 6G fluorescent solutions at femtoliter level from nano pipette is also achieved under the E-SEM environment. The results show that it is possible to perform small single cell injection using nano pipettes inside the E-SEM. {\textcopyright} 2009 IEEE NANO Organizers.",
keywords = "Environmental-SEM, Nano pipette injection, Nanomanipulation, Single cell analysis, Yeast cells",
author = "Yajing Shen and Masahiro Nakajima and Ahmad, \{Mohd Ridzuan\} and Toshio Fukuda and Seiji Kojima and Michio Homma",
year = "2009",
month = jul,
language = "English",
isbn = "978-1-4244-4832-6",
publisher = "IEEE",
pages = "518--521",
booktitle = "2009 9th IEEE Conference on Nanotechnology",
address = "United States",
note = "9th IEEE Conference on Nanotechnology (IEEE NANO 2009) ; Conference date: 26-07-2009 Through 30-07-2009",
}