Single cell injection using nano pipette via nanorobotic manipulation system inside E-SEM

Yajing Shen, Masahiro Nakajima, Mohd Ridzuan Ahmad, Toshio Fukuda, Seiji Kojima, Michio Homma

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review

17 Citations (Scopus)

Abstract

In this work, a nano injection system for single cell with small size (diameter: -5 μm) is proposed. An environmental scanning electron microscope (E-SEM) nanorobotic manipulation system has been constructed with three units and seven degrees of freedom (DOFs) in total. It can provide a real-time observation with nanoscale resolution during the experiment. Micro pulling and FIB etching are used to control the tip size of nano pipette accurately. In situ penetration of single wild-type yeast cell (W303) is performed using nano pipette inside the E-SEM based on the nanorobotic manipulation system. Spouting experiment of Rhodamine 6G fluorescent solutions at femtoliter level from nano pipette is also achieved under the E-SEM environment. The results show that it is possible to perform small single cell injection using nano pipettes inside the E-SEM. © 2009 IEEE NANO Organizers.
Original languageEnglish
Title of host publication2009 9th IEEE Conference on Nanotechnology, IEEE NANO 2009
Pages518-521
Publication statusPublished - 2009
Externally publishedYes
Event2009 9th IEEE Conference on Nanotechnology, IEEE NANO 2009 - Genoa, Italy
Duration: 26 Jul 200930 Jul 2009

Conference

Conference2009 9th IEEE Conference on Nanotechnology, IEEE NANO 2009
Country/TerritoryItaly
CityGenoa
Period26/07/0930/07/09

Research Keywords

  • Environmental-SEM
  • Nano pipette injection
  • Nanomanipulation
  • Single cell analysis
  • Yeast cells

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