Abstract
In this work, a nano injection system for single cell with small size (diameter: -5 μm) is proposed. An environmental scanning electron microscope (E-SEM) nanorobotic manipulation system has been constructed with three units and seven degrees of freedom (DOFs) in total. It can provide a real-time observation with nanoscale resolution during the experiment. Micro pulling and FIB etching are used to control the tip size of nano pipette accurately. In situ penetration of single wild-type yeast cell (W303) is performed using nano pipette inside the E-SEM based on the nanorobotic manipulation system. Spouting experiment of Rhodamine 6G fluorescent solutions at femtoliter level from nano pipette is also achieved under the E-SEM environment. The results show that it is possible to perform small single cell injection using nano pipettes inside the E-SEM. © 2009 IEEE NANO Organizers.
Original language | English |
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Title of host publication | 2009 9th IEEE Conference on Nanotechnology, IEEE NANO 2009 |
Pages | 518-521 |
Publication status | Published - 2009 |
Externally published | Yes |
Event | 2009 9th IEEE Conference on Nanotechnology, IEEE NANO 2009 - Genoa, Italy Duration: 26 Jul 2009 → 30 Jul 2009 |
Conference
Conference | 2009 9th IEEE Conference on Nanotechnology, IEEE NANO 2009 |
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Country/Territory | Italy |
City | Genoa |
Period | 26/07/09 → 30/07/09 |
Research Keywords
- Environmental-SEM
- Nano pipette injection
- Nanomanipulation
- Single cell analysis
- Yeast cells