@inbook{370b546d08f04afcbfbe7a80cd8a7160,
title = "Single Cell Deposition",
abstract = "This chapter describes an experimental protocol for single cell pick-place using a general-purposed micromanipulator and a micropipette. The micromanipulation system uses computer vision and motion control approaches to accurately pick up a single cell, transfer it to a desired substrate/location, and deposit it at a specified location. A traditional glass micropipette having a diameter larger than the cell is used to aspirate a whole cell into the micropipette for pick-place. This protocol is designed to manipulate and position single cells within a variety of microenvironments. The system is broadly applicable to any type of substrate and can manipulate multiple cell types in an end-user customizable manner. The presented protocol is for aspirating and transferring single cells after trypsinization. Additional steps are required for pick-place cells in their adherent state, such as local trypsinization or mechanical cutting. The tool for pick-place of cells can be useful as a stand-alone or bolstering technology for single-cell studies. ",
keywords = "Cell deposition, Contact detection, Device fabrication, Passaging cells, Random deposition",
author = "Jun Liu and Christopher Moraes and Zhe Lu and Craig Simmons and Yu Sun",
year = "2012",
month = nov,
day = "28",
doi = "10.1016/B978-0-12-405914-6.00023-8",
language = "English",
isbn = "9780124059146",
series = "Laboratory methods in cell biology",
publisher = "Elsevier/Academic Press",
pages = "403--420",
editor = "Conn, {P. Michael}",
booktitle = "Methods in Cell Biology",
}