SINGLE-CELL CYTOKINE DETECTION VIA CELL-ANCHORED CAPTURE MATRIX BASED ON DROPLET MICROFLUIDICS

Ying XU; Chia-Hung CHEN

SINGLE-CELL CYTOKINE DETECTION VIA CELL-ANCHORED CAPTURE MATRIX BASED ON DROPLET MICROFLUIDICS

Department of Biomedical Engineering

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

Abstract

A universal droplet single-cell secretion assay is developed via cell-anchored capture matrix technology. Here, to demonstrate the capability of this assay, IL-16 cytokines produced by single cells were evaluated as an example. The cytokines secreted from the Raji cells can be determined via a flow cytometer, suggesting its application in
cytokines detection at high throughput.

Original language	English
Title of host publication	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	1645-1646
ISBN (Print)	978-1-7334190-3-1
Publication status	Published - Oct 2021
Event	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021) - Hybrid, Palm Springs, United States Duration: 10 Oct 2021 → 14 Oct 2021 https://microtas2021.org https://www.cbmsociety.org/conference/proceedings_archive.html http://www.microtas2021.org

Publication series

Name	MicroTAS - International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021)
Abbreviated title	MicroTAS 2021
Place	United States
City	Palm Springs
Period	10/10/21 → 14/10/21
Internet address	https://microtas2021.org https://www.cbmsociety.org/conference/proceedings_archive.html http://www.microtas2021.org

Research Keywords

Single cell analysis
Droplet barcodes
Capture matrix
Cytokines
Cellular secretion

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XU, Y., & CHEN, C.-H. (2021). SINGLE-CELL CYTOKINE DETECTION VIA CELL-ANCHORED CAPTURE MATRIX BASED ON DROPLET MICROFLUIDICS. In 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1645-1646). (MicroTAS - International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.

XU, Ying ; CHEN, Chia-Hung. / SINGLE-CELL CYTOKINE DETECTION VIA CELL-ANCHORED CAPTURE MATRIX BASED ON DROPLET MICROFLUIDICS. 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. pp. 1645-1646 (MicroTAS - International Conference on Miniaturized Systems for Chemistry and Life Sciences).

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title = "SINGLE-CELL CYTOKINE DETECTION VIA CELL-ANCHORED CAPTURE MATRIX BASED ON DROPLET MICROFLUIDICS",

abstract = "A universal droplet single-cell secretion assay is developed via cell-anchored capture matrix technology. Here, to demonstrate the capability of this assay, IL-16 cytokines produced by single cells were evaluated as an example. The cytokines secreted from the Raji cells can be determined via a flow cytometer, suggesting its application in cytokines detection at high throughput.",

keywords = "Single cell analysis, Droplet barcodes, Capture matrix, Cytokines, Cellular secretion",

author = "Ying XU and Chia-Hung CHEN",

year = "2021",

month = oct,

language = "English",

isbn = "978-1-7334190-3-1",

series = "MicroTAS - International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Chemical and Biological Microsystems Society",

pages = "1645--1646",

booktitle = "25th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

note = "25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021), MicroTAS 2021 ; Conference date: 10-10-2021 Through 14-10-2021",

url = "https://microtas2021.org, https://www.cbmsociety.org/conference/proceedings\_archive.html, http://www.microtas2021.org",

}

XU, Y & CHEN, C-H 2021, SINGLE-CELL CYTOKINE DETECTION VIA CELL-ANCHORED CAPTURE MATRIX BASED ON DROPLET MICROFLUIDICS. in 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. MicroTAS - International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 1645-1646, 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021), Palm Springs, California, United States, 10/10/21.

SINGLE-CELL CYTOKINE DETECTION VIA CELL-ANCHORED CAPTURE MATRIX BASED ON DROPLET MICROFLUIDICS. / XU, Ying ; CHEN, Chia-Hung.
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. p. 1645-1646 (MicroTAS - International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - SINGLE-CELL CYTOKINE DETECTION VIA CELL-ANCHORED CAPTURE MATRIX BASED ON DROPLET MICROFLUIDICS

AU - XU, Ying

AU - CHEN, Chia-Hung

PY - 2021/10

Y1 - 2021/10

N2 - A universal droplet single-cell secretion assay is developed via cell-anchored capture matrix technology. Here, to demonstrate the capability of this assay, IL-16 cytokines produced by single cells were evaluated as an example. The cytokines secreted from the Raji cells can be determined via a flow cytometer, suggesting its application in cytokines detection at high throughput.

AB - A universal droplet single-cell secretion assay is developed via cell-anchored capture matrix technology. Here, to demonstrate the capability of this assay, IL-16 cytokines produced by single cells were evaluated as an example. The cytokines secreted from the Raji cells can be determined via a flow cytometer, suggesting its application in cytokines detection at high throughput.

KW - Single cell analysis

KW - Droplet barcodes

KW - Capture matrix

KW - Cytokines

KW - Cellular secretion

UR - https://www.scopus.com/pages/publications/85137007236

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85137007236&origin=recordpage

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 978-1-7334190-3-1

T3 - MicroTAS - International Conference on Miniaturized Systems for Chemistry and Life Sciences

SP - 1645

EP - 1646

BT - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021)

Y2 - 10 October 2021 through 14 October 2021

ER -

SINGLE-CELL CYTOKINE DETECTION VIA CELL-ANCHORED CAPTURE MATRIX BASED ON DROPLET MICROFLUIDICS

Abstract

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Research Keywords

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