SINGLE ADHERENT CELL PROTEIN SECRETION ANALYSIS THROUGH DROPLET ENCAPSULATIONS OF HYDROGEL PARTICLES

M. Wang; E. X. Ng; H. L. Leo; C. T. Lim; C. H. Chen

SINGLE ADHERENT CELL PROTEIN SECRETION ANALYSIS THROUGH DROPLET ENCAPSULATIONS OF HYDROGEL PARTICLES

M. Wang
, E. X. Ng
, H. L. Leo
, C. T. Lim
, C. H. Chen^*

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

Abstract

Single adherent cell analysis is essential to understand bio-processes in physiological systems. It is useful in discovering cause-and-effect relationship in cancer research given the vast heterogeneity and frequent cross-communication among sub-population during epithelial mesenchymal transition (EMT). At present, there is yet an effective and efficient method in analysing single adhered cell. In this study we present a technique in detecting protein secretion through encapsulation of hydrogel particles with single cell adhered on. This method ensures accurate chemical isolation and substance stuffiness controls of individual cells.

Original language	English
Title of host publication	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)
Publisher	Chemical and Biological Microsystems Society
Pages	375-376
ISBN (Print)	978-1-5108-3416-3
Publication status	Published - Oct 2016
Externally published	Yes
Event	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016) - Convention Center Dublin, Dublin, Ireland Duration: 9 Oct 2016 → 13 Oct 2016 http://docplayer.net/51234665-Conference-at-a-glance.html http://www.microtas2016.org/

Conference

Conference	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)
Abbreviated title	MicroTAS 2016
Place	Ireland
City	Dublin
Period	9/10/16 → 13/10/16
Internet address	http://docplayer.net/51234665-Conference-at-a-glance.html http://www.microtas2016.org/

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Keywords

Hydrogel particles
Protease secretion analysis
Single adherent cell assay

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@inproceedings{0cfcd0aa73854f39bd4228d90293f2f7,

title = "SINGLE ADHERENT CELL PROTEIN SECRETION ANALYSIS THROUGH DROPLET ENCAPSULATIONS OF HYDROGEL PARTICLES",

abstract = "Single adherent cell analysis is essential to understand bio-processes in physiological systems. It is useful in discovering cause-and-effect relationship in cancer research given the vast heterogeneity and frequent cross-communication among sub-population during epithelial mesenchymal transition (EMT). At present, there is yet an effective and efficient method in analysing single adhered cell. In this study we present a technique in detecting protein secretion through encapsulation of hydrogel particles with single cell adhered on. This method ensures accurate chemical isolation and substance stuffiness controls of individual cells.",

keywords = "Hydrogel particles, Protease secretion analysis, Single adherent cell assay",

author = "M. Wang and Ng, \{E. X.\} and Leo, \{H. L.\} and Lim, \{C. T.\} and Chen, \{C. H.\}",

year = "2016",

month = oct,

language = "English",

isbn = "978-1-5108-3416-3 ",

pages = "375--376",

booktitle = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)",

publisher = "Chemical and Biological Microsystems Society",

note = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016), MicroTAS 2016 ; Conference date: 09-10-2016 Through 13-10-2016",

url = "http://docplayer.net/51234665-Conference-at-a-glance.html, http://www.microtas2016.org/",

}

Wang, M, Ng, EX, Leo, HL, Lim, CT & Chen, CH 2016, SINGLE ADHERENT CELL PROTEIN SECRETION ANALYSIS THROUGH DROPLET ENCAPSULATIONS OF HYDROGEL PARTICLES. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016). Chemical and Biological Microsystems Society, pp. 375-376, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016), Dublin, Ireland, 9/10/16.

SINGLE ADHERENT CELL PROTEIN SECRETION ANALYSIS THROUGH DROPLET ENCAPSULATIONS OF HYDROGEL PARTICLES. / Wang, M.; Ng, E. X.; Leo, H. L. et al.
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016). Chemical and Biological Microsystems Society, 2016. p. 375-376.

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - SINGLE ADHERENT CELL PROTEIN SECRETION ANALYSIS THROUGH DROPLET ENCAPSULATIONS OF HYDROGEL PARTICLES

AU - Wang, M.

AU - Ng, E. X.

AU - Leo, H. L.

AU - Lim, C. T.

AU - Chen, C. H.

PY - 2016/10

Y1 - 2016/10

N2 - Single adherent cell analysis is essential to understand bio-processes in physiological systems. It is useful in discovering cause-and-effect relationship in cancer research given the vast heterogeneity and frequent cross-communication among sub-population during epithelial mesenchymal transition (EMT). At present, there is yet an effective and efficient method in analysing single adhered cell. In this study we present a technique in detecting protein secretion through encapsulation of hydrogel particles with single cell adhered on. This method ensures accurate chemical isolation and substance stuffiness controls of individual cells.

AB - Single adherent cell analysis is essential to understand bio-processes in physiological systems. It is useful in discovering cause-and-effect relationship in cancer research given the vast heterogeneity and frequent cross-communication among sub-population during epithelial mesenchymal transition (EMT). At present, there is yet an effective and efficient method in analysing single adhered cell. In this study we present a technique in detecting protein secretion through encapsulation of hydrogel particles with single cell adhered on. This method ensures accurate chemical isolation and substance stuffiness controls of individual cells.

KW - Hydrogel particles

KW - Protease secretion analysis

KW - Single adherent cell assay

UR - https://www.scopus.com/pages/publications/85014147222

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85014147222&origin=recordpage

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 978-1-5108-3416-3

SP - 375

EP - 376

BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)

PB - Chemical and Biological Microsystems Society

T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)

Y2 - 9 October 2016 through 13 October 2016

ER -

SINGLE ADHERENT CELL PROTEIN SECRETION ANALYSIS THROUGH DROPLET ENCAPSULATIONS OF HYDROGEL PARTICLES

Abstract

Conference

UN SDGs

Research Keywords

Other Access Options

Permanent Link

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