Simultaneous optical and fluorescent microscopic measurement of drug retention in single cancer cells

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review

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Author(s)

Detail(s)

Original languageEnglish
Title of host publication2008 IEEE 14th International Mixed-Signals, Sensors, and Systems Test Workshop, IMS3TW
Publication statusPublished - 2008
Externally publishedYes

Publication series

Name2008 IEEE 14th International Mixed-Signals, Sensors, and Systems Test Workshop, IMS3TW

Conference

Title2008 IEEE 14th International Mixed-Signals, Sensors, and Systems Test Workshop, IMS3TW
PlaceCanada
CityVancouver, BC
Period18 - 20 June 2008

Abstract

A single cancer cell was isolated and captured by a microfluidic single-cell biochip. The chip was made of glass and was fabricated by standard photolithography and wet etch process. Simultaneous optical observation and fluorescent measurement were achieved on the captured cancer cell. This was achieved by using red light to observe the cell; whereas the green fluorescent emission was measured photometrically to monitor the drug concentration. A CCD camera was used to image the cell morphology. At the same time, a PMT detector was used to monitor the cellular drug concentrations change in the single cell as a decrease in the cellular fluorescence intensity. Kinetic information of drug concentration change was obtained by analyzing the real time PMT data. This experiment revealed that during the efflux process, the anti-cancer drug (daunorubicin, DNR) decreased as the drug was pumped out of the cell because of multidrug resistance. The reversal effect of a multidrug resistance inhibitor (Sodium orthovanadate, OVN) on the cellular drug retention in single cancer cells was examined in this study. It was found that a higher cellular DNR concentration was resulted when OVN was present. ©2008 Crown.

Bibliographic Note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to lbscholars@cityu.edu.hk.

Citation Format(s)

Simultaneous optical and fluorescent microscopic measurement of drug retention in single cancer cells. / Chen, Yuchun; Li, Paul C. H.

2008 IEEE 14th International Mixed-Signals, Sensors, and Systems Test Workshop, IMS3TW. 2008. 4581629 (2008 IEEE 14th International Mixed-Signals, Sensors, and Systems Test Workshop, IMS3TW).

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review