Simultaneous optical and fluorescent microscopic measurement of drug retention in single cancer cells

Yuchun Chen; Paul C. H. Li

doi:10.1109/IMS3TW.2008.4581629

Simultaneous optical and fluorescent microscopic measurement of drug retention in single cancer cells

Yuchun Chen
, Paul C. H. Li

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

2 Citations (Scopus)

Abstract

A single cancer cell was isolated and captured by a microfluidic single-cell biochip. The chip was made of glass and was fabricated by standard photolithography and wet etch process. Simultaneous optical observation and fluorescent measurement were achieved on the captured cancer cell. This was achieved by using red light to observe the cell; whereas the green fluorescent emission was measured photometrically to monitor the drug concentration. A CCD camera was used to image the cell morphology. At the same time, a PMT detector was used to monitor the cellular drug concentrations change in the single cell as a decrease in the cellular fluorescence intensity. Kinetic information of drug concentration change was obtained by analyzing the real time PMT data. This experiment revealed that during the efflux process, the anti-cancer drug (daunorubicin, DNR) decreased as the drug was pumped out of the cell because of multidrug resistance. The reversal effect of a multidrug resistance inhibitor (Sodium orthovanadate, OVN) on the cellular drug retention in single cancer cells was examined in this study. It was found that a higher cellular DNR concentration was resulted when OVN was present. ©2008 Crown.

Original language	English
Title of host publication	2008 IEEE 14th International Mixed-Signals, Sensors, and Systems Test Workshop, IMS3TW
DOIs	https://doi.org/10.1109/IMS3TW.2008.4581629
Publication status	Published - 2008
Externally published	Yes
Event	2008 IEEE 14th International Mixed-Signals, Sensors, and Systems Test Workshop, IMS3TW - Vancouver, BC, Canada Duration: 18 Jun 2008 → 20 Jun 2008

Publication series

Name	2008 IEEE 14th International Mixed-Signals, Sensors, and Systems Test Workshop, IMS3TW

Conference

Conference	2008 IEEE 14th International Mixed-Signals, Sensors, and Systems Test Workshop, IMS3TW
Place	Canada
City	Vancouver, BC
Period	18/06/08 → 20/06/08

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1109/IMS3TW.2008.4581629

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Chen, Y., & Li, P. C. H. (2008). Simultaneous optical and fluorescent microscopic measurement of drug retention in single cancer cells. In 2008 IEEE 14th International Mixed-Signals, Sensors, and Systems Test Workshop, IMS3TW Article 4581629 (2008 IEEE 14th International Mixed-Signals, Sensors, and Systems Test Workshop, IMS3TW). https://doi.org/10.1109/IMS3TW.2008.4581629

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title = "Simultaneous optical and fluorescent microscopic measurement of drug retention in single cancer cells",

abstract = "A single cancer cell was isolated and captured by a microfluidic single-cell biochip. The chip was made of glass and was fabricated by standard photolithography and wet etch process. Simultaneous optical observation and fluorescent measurement were achieved on the captured cancer cell. This was achieved by using red light to observe the cell; whereas the green fluorescent emission was measured photometrically to monitor the drug concentration. A CCD camera was used to image the cell morphology. At the same time, a PMT detector was used to monitor the cellular drug concentrations change in the single cell as a decrease in the cellular fluorescence intensity. Kinetic information of drug concentration change was obtained by analyzing the real time PMT data. This experiment revealed that during the efflux process, the anti-cancer drug (daunorubicin, DNR) decreased as the drug was pumped out of the cell because of multidrug resistance. The reversal effect of a multidrug resistance inhibitor (Sodium orthovanadate, OVN) on the cellular drug retention in single cancer cells was examined in this study. It was found that a higher cellular DNR concentration was resulted when OVN was present. {\textcopyright}2008 Crown.",

author = "Yuchun Chen and Li, \{Paul C. H.\}",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].; 2008 IEEE 14th International Mixed-Signals, Sensors, and Systems Test Workshop, IMS3TW ; Conference date: 18-06-2008 Through 20-06-2008",

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doi = "10.1109/IMS3TW.2008.4581629",

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booktitle = "2008 IEEE 14th International Mixed-Signals, Sensors, and Systems Test Workshop, IMS3TW",

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Chen, Y & Li, PCH 2008, Simultaneous optical and fluorescent microscopic measurement of drug retention in single cancer cells. in 2008 IEEE 14th International Mixed-Signals, Sensors, and Systems Test Workshop, IMS3TW., 4581629, 2008 IEEE 14th International Mixed-Signals, Sensors, and Systems Test Workshop, IMS3TW, 2008 IEEE 14th International Mixed-Signals, Sensors, and Systems Test Workshop, IMS3TW, Vancouver, BC, Canada, 18/06/08. https://doi.org/10.1109/IMS3TW.2008.4581629

Simultaneous optical and fluorescent microscopic measurement of drug retention in single cancer cells. / Chen, Yuchun; Li, Paul C. H.
2008 IEEE 14th International Mixed-Signals, Sensors, and Systems Test Workshop, IMS3TW. 2008. 4581629 (2008 IEEE 14th International Mixed-Signals, Sensors, and Systems Test Workshop, IMS3TW).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - Simultaneous optical and fluorescent microscopic measurement of drug retention in single cancer cells

AU - Chen, Yuchun

AU - Li, Paul C. H.

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2008

Y1 - 2008

N2 - A single cancer cell was isolated and captured by a microfluidic single-cell biochip. The chip was made of glass and was fabricated by standard photolithography and wet etch process. Simultaneous optical observation and fluorescent measurement were achieved on the captured cancer cell. This was achieved by using red light to observe the cell; whereas the green fluorescent emission was measured photometrically to monitor the drug concentration. A CCD camera was used to image the cell morphology. At the same time, a PMT detector was used to monitor the cellular drug concentrations change in the single cell as a decrease in the cellular fluorescence intensity. Kinetic information of drug concentration change was obtained by analyzing the real time PMT data. This experiment revealed that during the efflux process, the anti-cancer drug (daunorubicin, DNR) decreased as the drug was pumped out of the cell because of multidrug resistance. The reversal effect of a multidrug resistance inhibitor (Sodium orthovanadate, OVN) on the cellular drug retention in single cancer cells was examined in this study. It was found that a higher cellular DNR concentration was resulted when OVN was present. ©2008 Crown.

AB - A single cancer cell was isolated and captured by a microfluidic single-cell biochip. The chip was made of glass and was fabricated by standard photolithography and wet etch process. Simultaneous optical observation and fluorescent measurement were achieved on the captured cancer cell. This was achieved by using red light to observe the cell; whereas the green fluorescent emission was measured photometrically to monitor the drug concentration. A CCD camera was used to image the cell morphology. At the same time, a PMT detector was used to monitor the cellular drug concentrations change in the single cell as a decrease in the cellular fluorescence intensity. Kinetic information of drug concentration change was obtained by analyzing the real time PMT data. This experiment revealed that during the efflux process, the anti-cancer drug (daunorubicin, DNR) decreased as the drug was pumped out of the cell because of multidrug resistance. The reversal effect of a multidrug resistance inhibitor (Sodium orthovanadate, OVN) on the cellular drug retention in single cancer cells was examined in this study. It was found that a higher cellular DNR concentration was resulted when OVN was present. ©2008 Crown.

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U2 - 10.1109/IMS3TW.2008.4581629

DO - 10.1109/IMS3TW.2008.4581629

M3 - RGC 32 - Refereed conference paper (with host publication)

T3 - 2008 IEEE 14th International Mixed-Signals, Sensors, and Systems Test Workshop, IMS3TW

BT - 2008 IEEE 14th International Mixed-Signals, Sensors, and Systems Test Workshop, IMS3TW

T2 - 2008 IEEE 14th International Mixed-Signals, Sensors, and Systems Test Workshop, IMS3TW

Y2 - 18 June 2008 through 20 June 2008

ER -

Simultaneous optical and fluorescent microscopic measurement of drug retention in single cancer cells

Abstract

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Bibliographical note

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