Simultaneous determination of ten aminoglycoside antibiotics in aquatic feeds by high-performance liquid chromatography quadrupole-orbitrap mass spectrometry with pass-through cleanup

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

11 Scopus Citations
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Author(s)

  • Xiaoyi Lou
  • Yunyu Tang
  • Changling Fang
  • Cong Kong
  • Huijuan Yu
  • And 4 others
  • Yongfu Shi
  • Dongmei Huang
  • Yaoguang Guo
  • Dongxue Xiao

Related Research Unit(s)

Detail(s)

Original languageEnglish
Pages (from-to)324-333
Journal / PublicationChirality
Volume32
Issue number3
Online published26 Dec 2019
Publication statusPublished - Mar 2020

Abstract

A simple and sensitive method has been established based on pass-through cleanup and high-performance liquid chromatography quadrupole-orbitrap mass spectrometry (HPLC-Q/Orbitrap MS) for the simultaneous determination of ten aminoglycosides (AGs) in aquatic feeds. The extraction solution and cleanup procedure had been optimized, and good sensitivity, accuracy, and precision were obtained. The calibration curves of AGs were linearity (R2 > 0.99) in the range of 2.0 to 200 mu g/L (or 5.0 to 500 mu g/L). The limits of detection of AGs were between 10 and 25 mu g/kg. The recoveries of AGs ranged from 74.9% to 94.3%, and the intraday and interday relative standard deviations were less than 15%. Finally, this method was successfully applied to determine ten AGs in 30 aquatic feed samples. It might be the first time to use pass-through cleanup approach combined with HPLC-Q/Orbitrap MS method for AGs determination in aquatic feed samples.

Research Area(s)

  • antibiotic residues, exploration of detection method, instrumental analysis, modified solid phase extraction, optimization

Citation Format(s)

Simultaneous determination of ten aminoglycoside antibiotics in aquatic feeds by high-performance liquid chromatography quadrupole-orbitrap mass spectrometry with pass-through cleanup. / Lou, Xiaoyi; Tang, Yunyu; Fang, Changling et al.
In: Chirality, Vol. 32, No. 3, 03.2020, p. 324-333.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review