Silicon nanowire-based molecular beacons for high-sensitivity and sequence-specific DNA multiplexed analysis

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Shao Su
  • Xinpan Wei
  • Yiling Zhong
  • Yuanyuan Guo
  • Yuanyuan Su
  • And 4 others
  • Qing Huang
  • Shuit-Tong Lee
  • Chunhai Fan
  • Yao He

Detail(s)

Original languageEnglish
Pages (from-to)2582-2590
Journal / PublicationACS Nano
Volume6
Issue number3
Publication statusPublished - 27 Mar 2012

Abstract

Nanomaterial-based molecular beacons (nanoMBs) have been extensively explored due to unique merits of nanostructures, including gold nanoparticle (AuNP)-, carbon nanotube (CNT)-, and graphene-based nanoMBs. Those nanoMBs are well-studied; however, they possess relatively poor salt stability or low specificity, limiting their wide applications. Here, we present a novel kind of multicolor silicon-based nanoMBs by using AuNP-decorated silicon nanowires as high-performance quenchers. Significantly, the nanoMBs feature robust stability in high-concentration (0.1 M) salt solution and wide-ranging temperature (10-80 °C), high quenching efficiency (>90%) for various fluorophores (e.g., FAM, Cy5, and ROX), and large surfaces for simultaneous assembly of different DNA strands. We further show that silicon-based nanoMBs are highly effective for sensitive and specific multidetection of DNA targets. The unprecedented advantages of silicon-based multicolor nanoMBs would bring new opportunities for challenging bioapplications, such as allele discrimination, early cancer diagnosis, and molecular engineering, etc. © 2012 American Chemical Society.

Research Area(s)

  • DNA, gold nanoparticles, molecular beacons, multianalysis, silicon nanowires

Citation Format(s)

Silicon nanowire-based molecular beacons for high-sensitivity and sequence-specific DNA multiplexed analysis. / Su, Shao; Wei, Xinpan; Zhong, Yiling et al.

In: ACS Nano, Vol. 6, No. 3, 27.03.2012, p. 2582-2590.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review