Sequencing of argentinated peptides by means of matrix-assisted laser desorption/ionization tandem mass spectrometry

Ivan K. Chu; David M. Cox; Xu Guo; Inga Kireeva; Tai-Chu Lau; John C. McDermott

doi:10.1021/ac0111006

Author(s)

Ivan K. Chu
David M. Cox
Xu Guo
Inga Kireeva
Tai-Chu Lau
And 1 others

John C. McDermott

Detail(s)

Original language	English
Pages (from-to)	2072-2082
Journal / Publication	Analytical Chemistry
Volume	74
Issue number	9
Publication status	Published - 1 May 2002

Link(s)

DOI	DOI https://doi.org/10.1021/ac0111006 Final Published version
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Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-0036558177&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(7511f051-9de1-4f1e-962a-f6824f0d84eb).html

Abstract

Argentinated peptide ions are formed in abundance under matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) conditions in the presence of Ag⁺ ions. These argentinated peptide ions are fragmented facilely under MALDI-MS/MS conditions to yield [b_n + OH + Ag]⁺, [b_n - H + Ag]⁺ and [a_n - H + Ag]⁺ ions that are indicative of the C-terminal sequence. These observations parallel those made earlier under electrospray MS conditions (Chu, I. K.; Guo, X.; Lau, T.-C.; Siu, K. W. M. Anal. Chem. 1999, 71, 2364-2372). A mixed protonated and argentinated tryptic peptide map was generated from 37 fmol of bovine serum albumin (BSA) using MALDI-MS. MALDI-MS/MS data from four argentinated peptides at a protein amount of 350 fmol unambiguously identified the protein as BSA. Sequence-tag analysis of two argentinated tryptic peptides was used to identify unambiguously myocyte enhancer factor 2A, which had been recombinantly expressed in a bacterial cell line.

Citation Format(s)

[ RIS ] [ BibTeX ]

Sequencing of argentinated peptides by means of matrix-assisted laser desorption/ionization tandem mass spectrometry. / Chu, Ivan K.; Cox, David M.; Guo, Xu et al.
In: Analytical Chemistry, Vol. 74, No. 9, 01.05.2002, p. 2072-2082.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

Chu, IK, Cox, DM, Guo, X, Kireeva, I, Lau, T-C & McDermott, JC 2002, 'Sequencing of argentinated peptides by means of matrix-assisted laser desorption/ionization tandem mass spectrometry', Analytical Chemistry, vol. 74, no. 9, pp. 2072-2082. https://doi.org/10.1021/ac0111006

Chu, I. K., Cox, D. M., Guo, X., Kireeva, I., Lau, T.-C., & McDermott, J. C. (2002). Sequencing of argentinated peptides by means of matrix-assisted laser desorption/ionization tandem mass spectrometry. Analytical Chemistry, 74(9), 2072-2082. https://doi.org/10.1021/ac0111006

Chu IK, Cox DM, Guo X, Kireeva I, Lau TC, McDermott JC. Sequencing of argentinated peptides by means of matrix-assisted laser desorption/ionization tandem mass spectrometry. Analytical Chemistry. 2002 May 1;74(9):2072-2082. doi: 10.1021/ac0111006

Chu, Ivan K. ; Cox, David M. ; Guo, Xu et al. / Sequencing of argentinated peptides by means of matrix-assisted laser desorption/ionization tandem mass spectrometry. In: Analytical Chemistry. 2002 ; Vol. 74, No. 9. pp. 2072-2082.