Abstract
Pathogens may hide inside immune cells to avoid the detection of human infectious diseases. We revealed that intracellular bacteria cause monocytes to become much stiffer and larger than healthy individuals. Hence, we introduced a label-free microchip that detects and quantifies monocytes with internalized pathogens (MIP) using inertial focusing. Different bacteria and clinical isolates were utilized to validate the device's functionality and set the infected sample threshold. The MIP platform can complete processing within 1.5 h. Our proof-of-concept shows that the MIP device has excellent prospects for diagnosing microbial infection.
| Original language | English |
|---|---|
| Title of host publication | MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 785-786 |
| ISBN (Print) | 978-1-7334190-3-1 |
| Publication status | Published - Oct 2021 |
| Event | 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021) - Hybrid, Palm Springs, United States Duration: 10 Oct 2021 → 14 Oct 2021 https://microtas2021.org https://www.cbmsociety.org/conference/proceedings_archive.html http://www.microtas2021.org |
Publication series
| Name | MicroTAS - International Conference on Miniaturized Systems for Chemistry and Life Sciences |
|---|
Conference
| Conference | 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021) |
|---|---|
| Abbreviated title | MicroTAS 2021 |
| Place | United States |
| City | Palm Springs |
| Period | 10/10/21 → 14/10/21 |
| Internet address |
Research Keywords
- Diagnosis
- Intracellular pathogens
- Label-free
- Microfluidics