Sensitive and simultaneous detection of different disease markers using multiplexed gold nanorods

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

24 Scopus Citations
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Author(s)

  • Haowen Huang
  • Fang Liu
  • Shaowen Huang
  • Shishan Yuan
  • Bo Liao
  • Shoujun Yi
  • Yunlong Zeng

Detail(s)

Original languageEnglish
Pages (from-to)108-114
Journal / PublicationAnalytica Chimica Acta
Volume755
Publication statusPublished - 28 Nov 2012

Abstract

A multiplexed bioanalytical assay is produced by incorporating two types of gold nanorods (GNRs). Besides retaining the desirable features of common GNRs LSPR sensors, this sensor is easy to fabricate and requires only a visible-NIR spectrometer for detection. This assay can simultaneously detect different acceptor-ligand pairs by choosing the proper GNRs possessing various LPWs in a wide detection wavelength range and can be developed into a high-throughput detection method. This bioanalytical assay allows easy detection of human serum specimens infected by S. japonicum and tuberculosis (TB) from human serum specimens (human serum/Tris-HCl buffer ratio=1:104) without the need for sample pretreatment. The technique is very sensitive compared to other standard methods such as indirect hemagglutination assays (IHA) that require a serum concentration ratio of larger than 1:20 and enzyme-linked immunosorbent assays (ELISA) requiring a ratio larger than 1:100. This methodology can be readily extended to other immunoassays to realize wider diagnostic applications. © 2012 Elsevier B.V.

Research Area(s)

  • Gold nanorod, Multiplex bioanalytical assay, Schistosoma japonicum, Tuberculosis

Citation Format(s)

Sensitive and simultaneous detection of different disease markers using multiplexed gold nanorods. / Huang, Haowen; Liu, Fang; Huang, Shaowen et al.
In: Analytica Chimica Acta, Vol. 755, 28.11.2012, p. 108-114.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review