Sensitive and selective ctDNA detection based on functionalized black phosphorus nanosheets

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Chi Huang
  • Shushu Hu
  • Xue Zhang
  • Haodong Cui
  • Lie Wu
  • Na Yang
  • Wenhua Zhou
  • Xue-Feng Yu

Detail(s)

Original languageEnglish
Article number112384
Journal / PublicationBiosensors and Bioelectronics
Volume165
Online published11 Jun 2020
Publication statusPublished - 1 Oct 2020

Abstract

Circulating tumor DNA (ctDNA) identification is one of the most meaningful approaches towards early cancer diagnosis. However, effective and practical methods for analyzing this emerging class of biomarkers are still lacking. In this work, a biosensor based on nitrophenyl functionalized black phosphorus nanosheets (NP-BPs) is fabricated for sensitive and selective detection of ctDNA. In this work, a nitrophenyl functionalized black phosphorus nanosheets (NP-BPs) biosensor is fabricated for sensitive and selective detection of ctDNA. Due to the successful nitrophenyl functionalization, the NP-BPs biosensor shows higher quenching efficiency and stronger affinity towards single-stranded DNA (ssDNA), as compared with double-stranded DNA (dsDNA). Therefore, the NP-BPs biosensor exhibits 5.4-fold fluorescence enhancement when dye-labelled ssDNA probe forms dsDNA in the presence of its specific ctDNA target. This biosensor exhibits a detection limit of 50 fM and a wide linear detection range of 50 fM-80 pM, provides reliable readout in a short time (15 min). Moreover, the NP-BPs-based biosensor could be applied to discriminate single nucleotide polymorphisms in clinical serum samples. It is envisioned that the NP-BPs-based sensing platform has great potentials in early cancer diagnosis and monitoring cancer progression.

Research Area(s)

  • Biosensor, Black phosphorus, ctDNA, Nitrophenyl, Surface functionalization, Two-dimensional materials

Citation Format(s)

Sensitive and selective ctDNA detection based on functionalized black phosphorus nanosheets. / Huang, Chi; Hu, Shushu; Zhang, Xue; Cui, Haodong; Wu, Lie; Yang, Na; Zhou, Wenhua; Chu, Paul K.; Yu, Xue-Feng.

In: Biosensors and Bioelectronics, Vol. 165, 112384, 01.10.2020.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review