Sensing and Approaching Toxic Arsenate by Shewanella putrefaciens CN-32

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Lei Cheng
  • Di Min
  • Dong-Feng Liu
  • Wen-Wei Li
  • Han-Qing Yu

Detail(s)

Original languageEnglish
Pages (from-to)14604-14611
Number of pages8
Journal / PublicationEnvironmental Science and Technology
Volume53
Issue number24
Online published20 Nov 2019
Publication statusPublished - 17 Dec 2019
Externally publishedYes

Abstract

Although arsenic at a high concentration imposes strong selective pressure on microbes, various microbes have been found to grow in As-rich environments. So far, little is known about how microbes can sense and move toward arsenate in the environment, and the underlying molecular mechanisms have not been revealed. Here, we report the chemotaxis response toward arsenate (As(V)) by Shewanella putrefaciens CN-32, a model dissimilatory metal-reducing bacterium (DMRB), and elucidate the mechanisms. We find that S. putrefaciens CN-32 exhibits a chemotactic behavior toward As(V) and diverse electron acceptors. To sense As(V), S. putrefaciens CN-32 requires functional arsenate respiratory reductase but does not depend on its metal-reducing-like respiratory pathway. We observe that such a sense is governed by an energy taxis mechanism and mediated by several methyl-accepting chemotaxis proteins (MCPs), rather than a specific MCP. Moreover, we reveal that the chemotactic signal transduction pathway is conserved in Shewanella, and histidine kinase and flagella-mediated motility are essential for taxis toward As(V). This work reverses the conventional view about arsenic as a chemotactic inhibitor to microbes by revealing the positive chemotaxis of Shewanella to As(V).

Citation Format(s)

Sensing and Approaching Toxic Arsenate by Shewanella putrefaciens CN-32. / Cheng, Lei; Min, Di; Liu, Dong-Feng; Li, Wen-Wei; Yu, Han-Qing.

In: Environmental Science and Technology, Vol. 53, No. 24, 17.12.2019, p. 14604-14611.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review